Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian 116024, People's Republic of China.
Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian 116024, People's Republic of China.
Int J Biol Macromol. 2018 Apr 1;109:1-11. doi: 10.1016/j.ijbiomac.2017.12.019. Epub 2017 Dec 6.
Excessive free radicals can cause oxidative damage to human tissues, which results in a variety of diseases. Therefore, the development of antioxidant materials is one of the great projects in biomedical field. In this work, antioxidant protocatechuic acid (PCA) monomers were grafted onto chitosan (CS) backbones to develop a PCA grafted chitosan (PCA-g-CS) antioxidant copolymer via the method of free radical-induced grafting reaction. The formation of covalent bonds between PCA and CS were confirmed by FTIR, H NMR, XRD and UV-vis. The antioxidant activity of PCA-g-CS was analyzed by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl radical scavenging assays. In addition, the cytotoxicity of PCA-g-CS on neuron-like rat phaeochromocytoma (PC12) cells was evaluated by using MTT assay. The neuroprotective effects against hydrogen peroxide (HO) and l-glutamic acid (GLU) induced apoptosis in PC12 cells were also investigated. Our results demonstrated that the PCA-g-CS antioxidant copolymer had the ability to scavenge DPPH and hydroxyl radical in vitro. Furthermore, the PCA-g-CS was biocompatible and had neuroprotective effects against free radical-induced apoptosis in PC12 cells. This PCA-g-CS copolymer is firstly synthesized for neuroprotection and the results suggest the PCA-g-CS may be a potential antioxidant material in the treatment of oxidative damage related diseases.
过量的自由基会对人体组织造成氧化损伤，从而导致多种疾病。因此，开发抗氧化材料是生物医学领域的重大项目之一。在这项工作中，通过自由基引发接枝反应的方法，将抗氧化剂原儿茶酸（PCA）单体接枝到壳聚糖（CS）主链上，开发了一种 PCA 接枝壳聚糖（PCA-g-CS）抗氧化共聚物。FTIR、H NMR、XRD 和 UV-vis 证实了 PCA 和 CS 之间形成了共价键。通过 2,2-二苯基-1-苦基肼（DPPH）和羟基自由基清除实验分析了 PCA-g-CS 的抗氧化活性。此外，还通过 MTT 测定法评估了 PCA-g-CS 对神经元样大鼠嗜铬细胞瘤（PC12）细胞的细胞毒性。还研究了 PCA-g-CS 对过氧化氢（HO）和 l-谷氨酸（GLU）诱导的 PC12 细胞凋亡的神经保护作用。结果表明，PCA-g-CS 抗氧化共聚物具有体外清除 DPPH 和羟基自由基的能力。此外，PCA-g-CS 具有生物相容性，并具有对抗自由基诱导的 PC12 细胞凋亡的神经保护作用。首次将该 PCA-g-CS 共聚物用于神经保护，结果表明 PCA-g-CS 可能是治疗氧化损伤相关疾病的潜在抗氧化材料。
