State Key Laboratory of Fine Chemicals, School of Chemical Engineering, Dalian University of Technology, Dalian 116024, China; Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian 116024, China.
State Key Laboratory of Fine Chemicals, School of Chemical Engineering, Dalian University of Technology, Dalian 116024, China; Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian 116024, China; Research & Educational Center for the Control Engineering of Translational Precision Medicine (R-ECCE-TPM), School of Biomedical Engineering, Faculty of Electronic Information and Electrical Engineering, Dalian University of Technology, Dalian 116024, China.
Carbohydr Polym. 2021 Jan 15;252:117210. doi: 10.1016/j.carbpol.2020.117210. Epub 2020 Oct 13.
In this study, protocatechuic acid (PCA) was grafted onto carboxymethyl chitosan (CMCS) via EDC/NHS to improve the antioxidant effect. The grafting ratio of PCA-g-CMCS conjugates could be controlled by adjusting the pH value and feed ratio of raw materials. The conjugates exhibited similar pH sensitivity to CMCS and showed dramatic enhancements of DPPH and ABTS radicals scavenging activities, total antioxidant capacity, reducing power, and Fe-chelating activity. Three-dimensional porous PCA-g-CMCS hydrogel was prepared by lyophilization and secondary cross-linking. The shaped hydrogel preserved its antioxidant activity, and the sustained release of PCA-containing degraded fragment from biodegradable hydrogel could be achieved with the aid of lysozyme in vitro (15 days). PCA-g-CMCS hydrogel also showed excellent biocompatibility and protective effect on HO-induced oxidative damage in SH-SY5Y cells. These results suggested that PCA-g-CMCS conjugates and its hydrogel would appear to be a promising oxidation-resistant material for applications such as drug release and tissue engineering.
在这项研究中，通过 EDC/NHS 将原儿茶酸（PCA）接枝到羧甲基壳聚糖（CMCS）上，以提高抗氧化效果。PCA-g-CMCS 缀合物的接枝率可以通过调节 pH 值和原料的进料比来控制。该缀合物对 CMCS 表现出相似的 pH 敏感性，并显示出 DPPH 和 ABTS 自由基清除活性、总抗氧化能力、还原能力和 Fe 螯合活性的显著增强。通过冻干和二次交联制备了具有三维多孔结构的 PCA-g-CMCS 水凝胶。成型水凝胶保留了其抗氧化活性，并且在体外（15 天）在溶菌酶的帮助下，可以实现含有 PCA 的降解片段的持续释放。PCA-g-CMCS 水凝胶还表现出良好的生物相容性和对 HO 诱导的 SH-SY5Y 细胞氧化损伤的保护作用。这些结果表明，PCA-g-CMCS 缀合物及其水凝胶有望成为一种有前途的抗氧化材料，可用于药物释放和组织工程等应用。
