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An improved method for directly sequencing PCR amplified material using dimethyl sulphoxide.

作者信息

Winship P R

机构信息

Sir William Dunn School of Pathology, University of Oxford, UK.

出版信息

Nucleic Acids Res. 1989 Feb 11;17(3):1266. doi: 10.1093/nar/17.3.1266.

DOI:10.1093/nar/17.3.1266
PMID:2922271
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC331767/
Abstract
摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84cc/331767/6dee788e3f3a/nar00212-0438-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84cc/331767/6dee788e3f3a/nar00212-0438-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84cc/331767/6dee788e3f3a/nar00212-0438-a.jpg

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本文引用的文献

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Biochemistry. 1985 Jul 2;24(14):3736-50. doi: 10.1021/bi00335a049.
2
DNA sequencing with chain-terminating inhibitors.使用链终止抑制剂的DNA测序。
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7. doi: 10.1073/pnas.74.12.5463.
结合直接PCR技术和毛细管电泳，构建一种易于操作且高度灵敏的虾类传染病检测系统。
Life (Basel). 2022 Feb 13;12(2):276. doi: 10.3390/life12020276.
4
Low dose dimethyl sulfoxide driven gross molecular changes have the potential to interfere with various cellular processes.低剂量二甲基亚砜驱动的总分子变化有可能干扰各种细胞过程。
Sci Rep. 2018 Oct 4;8(1):14828. doi: 10.1038/s41598-018-33234-z.
5
Template-Independent Enzymatic Oligonucleotide Synthesis (TiEOS): Its History, Prospects, and Challenges.非模板依赖的酶促寡核苷酸合成（TiEOS）：其历史、前景与挑战
Biochemistry. 2018 Mar 27;57(12):1821-1832. doi: 10.1021/acs.biochem.7b00937. Epub 2018 Mar 13.
6
The structural stability and catalytic activity of DNA and RNA oligonucleotides in the presence of organic solvents.在有机溶剂存在下DNA和RNA寡核苷酸的结构稳定性及催化活性。
Biophys Rev. 2016 Mar;8(1):11-23. doi: 10.1007/s12551-015-0188-0. Epub 2016 Jan 11.
7
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PLoS One. 2016 Jun 7;11(6):e0156478. doi: 10.1371/journal.pone.0156478. eCollection 2016.
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The Effect of Dimethyl Sulfoxide on Supercoiled DNA Relaxation Catalyzed by Type I Topoisomerases.二甲基亚砜对I型拓扑异构酶催化的超螺旋DNA松弛的影响。
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Gene Assembly from Chip-Synthesized Oligonucleotides.基于芯片合成寡核苷酸的基因组装
Curr Protoc Chem Biol. 2012 Mar 1;2012. doi: 10.1002/9780470559277.ch110190.
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