• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

来自厌氧真菌新美鞭菌属的富含AT的基因组DNA非常显著。

Remarkably AT-rich genomic DNA from the anaerobic fungus Neocallimastix.

作者信息

Brownlee A G

机构信息

CSIRO, Division of Animal Production, Blacktown, NSW, Australia.

出版信息

Nucleic Acids Res. 1989 Feb 25;17(4):1327-35. doi: 10.1093/nar/17.4.1327.

DOI:10.1093/nar/17.4.1327
PMID:2922283
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC331806/
Abstract

The genomic DNA of an anaerobic rumen phycomycete of the genus Neocallimastix has been purified and characterized. The non-repetitive fraction of the DNA has a G.C content of only 13%. The ribosomal RNA genes are highly reiterated, making up about 30% of the total DNA, and are evident as a more G.C-rich satellite with a repeating unit of about 9.4 kilobases (Kb). A.T-rich regions of DNA are highly dispersed and possess some sequence complexity. Chemical analysis of the DNA constituents reveals no evidence of modified bases. The genome of this anaerobic fungus has the highest A.T content of any organism so far described.

摘要

新美鞭菌属一种厌氧瘤胃藻状菌的基因组DNA已被纯化并进行了特性分析。该DNA的非重复部分的鸟嘌呤-胞嘧啶（G.C）含量仅为13%。核糖体RNA基因高度重复，约占总DNA的30%，并表现为一个富含G.C的卫星序列，其重复单元约为9.4千碱基（Kb）。富含腺嘌呤-胸腺嘧啶（A.T）的DNA区域高度分散且具有一定的序列复杂性。对DNA成分的化学分析未发现修饰碱基的证据。这种厌氧真菌的基因组是迄今为止所描述的所有生物体中A.T含量最高的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d03c/331806/797ca4b9b041/nar00213-0063-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d03c/331806/d35f113436e7/nar00213-0061-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d03c/331806/da998ca05262/nar00213-0062-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d03c/331806/797ca4b9b041/nar00213-0063-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d03c/331806/d35f113436e7/nar00213-0061-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d03c/331806/da998ca05262/nar00213-0062-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d03c/331806/797ca4b9b041/nar00213-0063-a.jpg

相似文献

1
Remarkably AT-rich genomic DNA from the anaerobic fungus Neocallimastix.来自厌氧真菌新美鞭菌属的富含AT的基因组DNA非常显著。
Nucleic Acids Res. 1989 Feb 25;17(4):1327-35. doi: 10.1093/nar/17.4.1327.
2
Cloning, sequencing, and expression of an endoglucanase gene from the rumen anaerobic fungus Neocallimastix frontalis MCH3.来自瘤胃厌氧真菌新美鞭菌属MCH3的一种内切葡聚糖酶基因的克隆、测序及表达
Biosci Biotechnol Biochem. 1998 Sep;62(9):1795-8. doi: 10.1271/bbb.62.1795.
3
[PCR amplification of anaerobic fungal 18S rDNA from landfill sites].[从垃圾填埋场进行厌氧真菌18S rDNA的聚合酶链反应扩增]
Sheng Wu Gong Cheng Xue Bao. 2001 Sep;17(5):515-9.
4
Sequence of the phosphoenolpyruvate carboxykinase-encoding cDNA from the rumen anaerobic fungus Neocallimastix frontalis: comparison of the amino acid sequence with animals and yeast.
Gene. 1992 Jan 2;110(1):57-63. doi: 10.1016/0378-1119(92)90444-t.
5
Characterization of the "promoter region" of the enolase-encoding gene enol from the anaerobic fungus Neocallimastix frontalis: sequence and promoter analysis.来自厌氧真菌新丽鞭毛虫的烯醇酶编码基因enol的“启动子区域”的特征：序列和启动子分析
Curr Genet. 1995 Jun;28(1):80-6. doi: 10.1007/BF00311885.
6
Molecular analysis of the anaerobic rumen fungus Orpinomyces - insights into an AT-rich genome.厌氧瘤胃真菌奥尔平氏菌的分子分析——对富含AT基因组的见解
Microbiology (Reading). 2005 Jan;151(Pt 1):121-133. doi: 10.1099/mic.0.27353-0.
7
Cloning and expression of multiple cellulase cDNAs from the anaerobic rumen fungus Neocallimastix patriciarum in Escherichia coli.从厌氧瘤胃真菌梨形新美鞭菌中克隆多个纤维素酶cDNA并在大肠杆菌中表达
J Gen Microbiol. 1992 Jul;138(7):1413-20. doi: 10.1099/00221287-138-7-1413.
8
Molecular evolution of the fungi: relationship of the Basidiomycetes, Ascomycetes, and Chytridiomycetes.真菌的分子进化：担子菌纲、子囊菌纲和壶菌纲之间的关系。
Mol Biol Evol. 1992 Mar;9(2):285-96. doi: 10.1093/oxfordjournals.molbev.a040720.
9
Ribosomal RNA cistrons in Allomyces arbuscula.丛枝异水霉中的核糖体RNA顺反子。
Biochim Biophys Acta. 1977 Oct 18;478(4):392-406. doi: 10.1016/0005-2787(77)90095-8.
10
DNA of some anaerobic rumen fungi: G + C content determination.某些厌氧瘤胃真菌的DNA：鸟嘌呤与胞嘧啶含量测定
FEMS Microbiol Lett. 1991 Aug 15;66(3):267-70. doi: 10.1016/0378-1097(91)90272-c.

引用本文的文献

1
Genomic and transcriptomic characterization of carbohydrate-active enzymes in the anaerobic fungus Neocallimastix cameroonii var. constans.厌氧真菌喀麦隆新丽鞭毛虫常量变种中碳水化合物活性酶的基因组和转录组特征分析
G3 (Bethesda). 2025 Aug 6;15(8). doi: 10.1093/g3journal/jkaf137.
2
Dynamic changes in the yak rumen eukaryotic community and metabolome characteristics in response to feed type.牦牛瘤胃真核生物群落的动态变化及对饲料类型的代谢组特征
Front Vet Sci. 2022 Dec 22;9:1027967. doi: 10.3389/fvets.2022.1027967. eCollection 2022.
3
Nucleotide and protein researches on anaerobic fungi during four decades.

本文引用的文献

1
Fluorometric determination of DNA in aquatic microorganisms by use of hoechst 33258.利用 Hoechst 33258 荧光光度法测定水中微生物的 DNA。
Appl Environ Microbiol. 1982 Jun;43(6):1393-9. doi: 10.1128/aem.43.6.1393-1399.1982.
2
Deoxyribonucleic acid base composition and taxonomy of some protozoa.
Nature. 1962 Nov 24;196:795-6. doi: 10.1038/196795a0.
3
On the genetic basis of variation and heterogeneity of DNA base composition.论DNA碱基组成的变异与异质性的遗传基础。
四十年来对厌氧真菌的核苷酸和蛋白质研究。
J Anim Sci Technol. 2020 Mar;62(2):121-140. doi: 10.5187/jast.2020.62.2.121. Epub 2020 Mar 31.
4
Genomic and proteomic biases inform metabolic engineering strategies for anaerobic fungi.基因组和蛋白质组学偏差为厌氧真菌的代谢工程策略提供了信息。
Metab Eng Commun. 2019 Nov 15;10:e00107. doi: 10.1016/j.mec.2019.e00107. eCollection 2020 Jun.
5
Assignment of virus and antimicrobial resistance genes to microbial hosts in a complex microbial community by combined long-read assembly and proximity ligation.通过组合长读长组装和邻近连接技术，对复杂微生物群落中的病毒和抗菌药物耐药基因进行微生物宿主的基因定位。
Genome Biol. 2019 Aug 2;20(1):153. doi: 10.1186/s13059-019-1760-x.
6
Plasmodium parasites of birds have the most AT-rich genes of eukaryotes.鸟类疟原虫拥有真核生物中 AT 含量最高的基因。
Microb Genom. 2018 Feb;4(2). doi: 10.1099/mgen.0.000150. Epub 2018 Jan 23.
7
Transcriptomic characterization of : a novel, non-rhizoid-forming lignocellulolytic anaerobic fungus.一种新型的、不形成根状菌索的木质纤维素分解厌氧真菌的转录组学特征
Biotechnol Biofuels. 2017 Dec 20;10:305. doi: 10.1186/s13068-017-0997-4. eCollection 2017.
8
Enabling large-scale next-generation sequence assembly with Blacklight.利用Blacklight实现大规模下一代序列组装。
Concurr Comput. 2014 Sep 10;26(13):2157-2166. doi: 10.1002/cpe.3231.
9
The genome of the anaerobic fungus Orpinomyces sp. strain C1A reveals the unique evolutionary history of a remarkable plant biomass degrader.厌氧真菌 Orpinomyces sp. 菌株 C1A 的基因组揭示了一种非凡的植物生物质降解菌的独特进化历史。
Appl Environ Microbiol. 2013 Aug;79(15):4620-34. doi: 10.1128/AEM.00821-13. Epub 2013 May 24.
10
Catalytic efficiency diversification of duplicate β-1,3-1,4-glucanases from Neocallimastix patriciarum J11.从 Neocallimastix patriciarum J11 中复制的β-1,3-1,4-葡聚糖酶的催化效率多样化。
Appl Environ Microbiol. 2012 Jun;78(12):4294-300. doi: 10.1128/AEM.07473-11. Epub 2012 Apr 6.
Proc Natl Acad Sci U S A. 1962 Apr 15;48(4):582-92. doi: 10.1073/pnas.48.4.582.
4
Determination of 5-methylcytosine from plant DNA by high-performance liquid chromatography.通过高效液相色谱法测定植物DNA中的5-甲基胞嘧啶
Biochim Biophys Acta. 1981 Jun 26;654(1):52-6. doi: 10.1016/0005-2787(81)90135-0.
5
A method for extracting high-molecular-weight deoxyribonucleic acid from fungi.一种从真菌中提取高分子量脱氧核糖核酸的方法。
Anal Biochem. 1982 Jan 1;119(1):158-63. doi: 10.1016/0003-2697(82)90680-7.
6
Sequence organization in Dictyostelium: unique structure at the 5'-ends of protein coding genes.盘基网柄菌中的序列组织：蛋白质编码基因5'端的独特结构
Nucleic Acids Res. 1983 Jan 25;11(2):541-52. doi: 10.1093/nar/11.2.541.
7
Rumen bacterial and fungal degradation of Digitaria pentzii grown with or without sulfur.有无硫条件下生长的俯仰马唐的瘤胃细菌和真菌降解
Appl Environ Microbiol. 1983 Sep;46(3):738-48. doi: 10.1128/aem.46.3.738-748.1983.
8
DNA base sequence complementarity and the definition of fungal taxa.
Microbiol Sci. 1984 May;1(2):44-8.
9
Quantitative determination of 5-methylcytosine in DNA by reverse-phase high-performance liquid chromatography.采用反相高效液相色谱法对DNA中的5-甲基胞嘧啶进行定量测定。
Anal Biochem. 1983 Nov;135(1):165-71. doi: 10.1016/0003-2697(83)90746-7.
10
Evolutionary relatedness of Plasmodium species as determined by the structure of DNA.通过DNA结构确定的疟原虫物种的进化相关性。
Science. 1984 Aug 24;225(4664):808-11. doi: 10.1126/science.6382604.