School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil.
School of Dentistry, University of Ribeirão Preto, Ribeirão Preto, Brazil.
Int Endod J. 2018 Jun;51(6):674-683. doi: 10.1111/iej.12883. Epub 2018 Jan 2.
To evaluate the effects of 2.8% or 10% calcium chloride (CaCl ) in calcium aluminate cement (CAC) with either bismuth oxide (Bi O ) or zinc oxide (ZnO) as radiopacifiers on the progression of osteogenic cell cultures.
Rat calvaria-derived cells were grown on Thermanox coverslips for 24 h and exposed to samples of (i) CACb: with 2.8% CaCl and 25% Bi O ; (ii) CACb+: with 10% CaCl and 25% Bi O ; (iii) CACz: with 2.8% CaCl and 25% ZnO; or (iv) CACz+: with 10% CaCl and 25% ZnO, placed on inserts. Nonexposed cultures served as the control. Calcium and phosphorus contents in culture media were quantified. The effects of the cements on cell apoptosis, cell viability and acquisition of the osteogenic cell phenotype were evaluated. Data were compared by Kruskal-Wallis test (α = 5%).
CACb+ promoted the highest levels of calcium in the culture media; CACz+, the lowest levels of phosphorus (P < 0.05). CACz+ and CACb increased cell apoptosis (P < 0.05). CACb reduced cell viability (P < 0.05) and the expression of the osteoblastic phenotype. CACz+ and CACb+ promoted greater cell differentiation and matrix mineralization compared to CACz and CACb (P < 0.05).
For CAC with the lower CaCl content, the use of Bi O was detrimental for osteoblastic cell survival and differentiation compared to ZnO, while CAC with the higher CaCl content supported the acquisition of the osteogenic cell phenotype in vitro regardless of the radiopacifier used. Thus, CAC with 10% CaCl would potentially promote bone repair in the context of endodontic therapies.
评估 2.8%或 10%氯化钙（CaCl）在含氧化铋（Bi2O3）或氧化锌（ZnO）的铝酸钙水泥（CAC）中的作用，这些 CAC 用作放射增感剂对成骨细胞培养物的进展的影响。
大鼠颅盖骨来源的细胞在 Thermanox 盖玻片上培养 24 小时，然后将其暴露于以下样品中：（i）CACb：含 2.8%CaCl 和 25%Bi2O3；（ii）CACb+：含 10%CaCl 和 25%Bi2O3；（iii）CACz：含 2.8%CaCl 和 25%ZnO；或（iv）CACz+：含 10%CaCl 和 25%ZnO，放置在插入物上。未暴露的培养物作为对照。定量测定培养基中的钙和磷含量。评估水泥对细胞凋亡、细胞活力和获得成骨细胞表型的影响。通过 Kruskal-Wallis 检验（α=5%）比较数据。
CACb+促进培养基中钙含量最高；CACz+，磷含量最低（P<0.05）。CACz+和 CACb 增加细胞凋亡（P<0.05）。CACb 降低细胞活力（P<0.05）并降低成骨细胞表型的表达。与 CACz 和 CACb 相比，CACz+和 CACb+促进了更大的细胞分化和基质矿化（P<0.05）。
对于 CaCl 含量较低的 CAC，与 ZnO 相比，使用 Bi2O3 不利于成骨细胞的存活和分化，而 CaCl 含量较高的 CAC 支持体外获得成骨细胞表型，而与使用的放射增感剂无关。因此，10%CaCl 的 CAC 可能会促进根管治疗中骨修复。
