Nozdrenko D M, Miroshnychenko M S, Soroca V M, Korchins ka L V, Zavodovskiy D O
Ukr Biochem J. 2016 Mar-Apr;88(2):82-8. doi: 10.15407/ubj88.02.082.
We investigated the effect of chlorpyrifos, an organophosphate insecticide, on Ca2+,Mg2+-ATPase activity of sarcoplasmic reticulum and on contraction dynamics (force and length changes) of Rana temporaria m. tibialis anterior muscle fiber bundles. All of the used concentrations of chlorpyrifos (10-6 to 10-5 M) caused decrease of Ca2+,Mg2+-ATPase activity. The inhibition of Ca2+,Mg2+-ATPase activity by chlorpyriphos in concentrations of 10-6 M to 7.5·10-6 M is due to permeation of sarcoplasmic reticulum rather than due to direct enzyme inhibition by organophosphate insecticides. The inhibitory properties of the compound were higher at increased concentration and exposure timeframes. Chlorpyrifos in concentration range of 10-6 to 7.5·10-6 M causes changes in muscle fiber response force that were more pronounced than changes in contractile length. We demonstrated inhibition of Ca2+,Mg2+-ATPase activity caused by noncholinergic effects of chlorpyriphos. It is possible to conclude that influence of organophosphate insecticides happens not only in the neuromuscular transmission but also on the level of subcellular structures.
我们研究了有机磷杀虫剂毒死蜱对欧洲林蛙胫前肌纤维束肌浆网Ca2 +、Mg2 + -ATP酶活性以及收缩动力学（力和长度变化）的影响。所有使用的毒死蜱浓度（10 - 6至10 - 5 M）均导致Ca2 +、Mg2 + -ATP酶活性降低。毒死蜱在10 - 6 M至7.5·10 - 6 M浓度下对Ca2 +、Mg2 + -ATP酶活性的抑制是由于肌浆网的通透性增加，而非有机磷杀虫剂对酶的直接抑制。该化合物的抑制特性在浓度和暴露时间增加时更高。浓度范围为10 - 6至7.5·10 - 6 M的毒死蜱引起的肌肉纤维反应力变化比收缩长度变化更明显。我们证明了毒死蜱的非胆碱能效应会导致Ca2 +、Mg2 + -ATP酶活性受到抑制。可以得出结论，有机磷杀虫剂的影响不仅发生在神经肌肉传递过程中，还发生在亚细胞结构水平上。
