Boström Johan, Sramkova Zuzana, Salašová Alena, Johard Helena, Mahdessian Diana, Fedr Radek, Marks Carolyn, Medalová Jiřina, Souček Karel, Lundberg Emma, Linnarsson Sten, Bryja Vítězslav, Sekyrova Petra, Altun Mikael, Andäng Michael
Science for Life Laboratory, Division of Translational Medicine and Chemical Biology, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.
Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden.
PLoS One. 2017 Dec 11;12(12):e0188772. doi: 10.1371/journal.pone.0188772. eCollection 2017.
The cell cycle coordinates core functions such as replication and cell division. However, cell-cycle-regulated transcription in the control of non-core functions, such as cell identity maintenance through specific transcription factors (TFs) and signalling pathways remains unclear. Here, we provide a resource consisting of mapped transcriptomes in unsynchronized HeLa and U2OS cancer cells sorted for cell cycle phase by Fucci reporter expression. We developed a novel algorithm for data analysis that enables efficient visualization and data comparisons and identified cell cycle synchronization of Notch signalling and TFs associated with development. Furthermore, the cell cycle synchronizes with the circadian clock, providing a possible link between developmental transcriptional networks and the cell cycle. In conclusion we find that cell cycle synchronized transcriptional patterns are temporally compartmentalized and more complex than previously anticipated, involving genes, which control cell identity and development.
细胞周期协调着诸如复制和细胞分裂等核心功能。然而，细胞周期调控的转录在非核心功能的控制中，比如通过特定转录因子（TFs）和信号通路维持细胞身份，仍不清楚。在这里，我们提供了一个资源，它由通过Fucci报告基因表达按细胞周期阶段分选的未同步化的HeLa和U2OS癌细胞中的映射转录组组成。我们开发了一种用于数据分析的新算法，该算法能够实现高效的可视化和数据比较，并确定了Notch信号通路和与发育相关的转录因子的细胞周期同步性。此外，细胞周期与生物钟同步，为发育转录网络和细胞周期之间提供了可能的联系。总之，我们发现细胞周期同步的转录模式在时间上是分区的，并且比之前预期的更复杂，涉及控制细胞身份和发育的基因。
