Wang Xiaoyan, Chen Guanghua, Huang Chao, Tu Hualei, Zou Jilong, Yan Jinglong
Department of Orthopedics, The Second Affiliated Hospital of Harbin Medical University, Harbin 150086, P.R. China.
Key Laboratory of the Ministry of Education, Ministry of Myocardial Ischemia, Harbin 150086, P.R. China.
Oncotarget. 2017 Oct 9;8(58):98336-98347. doi: 10.18632/oncotarget.21683. eCollection 2017 Nov 17.
The extracellular matrix(ECM), which is primarily composed of collagens and proteoglycans, plays a key role in cell proliferation, differentiation, and migration and interactions between cells. In this study, we produced chitosan/gelatin/bone marrow stem cells-derived extracellular matrix(C/G/BMSCs-dECM) scaffolds via lyophilization and cross-linking, and chitosan/gelatin(C/G) scaffolds were used as controls. For the C/G/BMSCs-dECM scaffolds, the average pore size was 289.17 ± 80.28 μm; the average porosity was 89.25 ± 3.75%; the average compressive modulus was 0.82 ± 0.07 MPa; and the average water uptake ratio was 13.81 ± 1.00. , the C/G/BMSCs-dECM scaffolds promoted bone marrow stem cells(BMSCs) attachment and proliferation. Moreover, improved osteogenic differentiation was observed for these scaffolds. Thus, C/G/BMSCs-dECM is a promising material for bone tissue engineering.
细胞外基质（ECM）主要由胶原蛋白和蛋白聚糖组成，在细胞增殖、分化、迁移以及细胞间相互作用中起关键作用。在本研究中，我们通过冻干和交联制备了壳聚糖/明胶/骨髓干细胞衍生的细胞外基质（C/G/BMSCs-dECM）支架，并将壳聚糖/明胶（C/G）支架用作对照。对于C/G/BMSCs-dECM支架，平均孔径为289.17±80.28μm；平均孔隙率为89.25±3.75%；平均压缩模量为0.82±0.07MPa；平均吸水率为13.81±1.00。C/G/BMSCs-dECM支架促进了骨髓干细胞（BMSCs）的附着和增殖。此外，观察到这些支架的成骨分化得到改善。因此，C/G/BMSCs-dECM是一种有前途的骨组织工程材料。
