Department of Anesthesiology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China.
Department of Anesthesiology, The Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.
Oxid Med Cell Longev. 2017;2017:9718615. doi: 10.1155/2017/9718615. Epub 2017 Nov 5.
Our recent study demonstrated that acetylcholinesterase (AChE) activity at the neuromuscular junction (NMJ) of the diaphragm decreased during sepsis. However, the mechanisms were not clearly identified. In this study, we aimed to investigate whether the decreased AChE activity was related to oxidative stress by observing AChE activity in different grades of sepsis induced by caecal ligation and puncture (CLP). At 24 h after surgery, an assay of thiobarbituric acid reactive species (TBARS) and protein carbonyls, as well as the myeloperoxidase (MPO), superoxide dismutase (SOD), and catalase (CAT) activity, was conducted. AChE activity was measured by biochemical and histological detection. AChE and CAT activity in the diaphragm decreased, while the contents of TBARS and protein carbonyls, the activity of MPO and SOD, and the SOD/CAT ratios increased. The above changes were much more significant in the mid-grade septic group than in the low-grade septic group. The colour of the AChE activity staining at the NMJ gradually lightened from the sham surgery group to the mid-grade septic group. AChE activity was significantly negatively correlated with the levels of TBARS and protein carbonyls. We consider that oxidative stress might be responsible for decreased AChE activity in the diaphragms of rats induced with sepsis.
我们最近的研究表明，在脓毒症期间膈肌神经肌肉接头（NMJ）的乙酰胆碱酯酶（AChE）活性降低。然而，其机制尚不清楚。在这项研究中，我们旨在通过观察盲肠结扎和穿刺（CLP）诱导的不同程度脓毒症中 AChE 活性，来研究 AChE 活性降低是否与氧化应激有关。术后 24 小时，进行硫代巴比妥酸反应性物质（TBARS）和蛋白质羰基的测定，以及髓过氧化物酶（MPO）、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）的活性。通过生化和组织学检测来测量 AChE 活性。膈肌中的 AChE 和 CAT 活性降低，而 TBARS 和蛋白质羰基的含量、MPO 和 SOD 的活性以及 SOD/CAT 比值增加。与低等级脓毒症组相比，中等级脓毒症组的上述变化更为明显。NMJ 处的 AChE 活性染色的颜色从假手术组逐渐变浅到中等级脓毒症组。AChE 活性与 TBARS 和蛋白质羰基的水平呈显著负相关。我们认为氧化应激可能是导致脓毒症大鼠膈肌 AChE 活性降低的原因。
