Centro de Biotecnología FEMSA, Escuela de Ingeniería y Ciencias, Tecnológico de Monterrey, Monterrey, NL, México.
Departamento de Biotecnología y Microbiología de Alimentos, Instituto de Investigación en Ciencias de la Alimentación CIAL (CSIC-UAM), Madrid, Spain.
J Sci Food Agric. 2018 Jul;98(9):3246-3254. doi: 10.1002/jsfa.8827. Epub 2018 Jan 22.
Addition of persimmon fruit, which is highly rich in carotenoids, to dairy products represents an alternative to obtain functional foods. However, carotenoid bioaccessibility is strongly influenced by fat content and food composition. That is why in vitro bioaccessibility of individual carotenoids was evaluated in persimmon-based dairy products formulated with whole (3.6% fat) or skimmed milk (0.25% fat) and different freeze-dried persimmon tissues.
Unambiguous identification of seven xanthophylls (neoxanthin, violaxanthin, antheraxanthin, lutein, zeaxanthin, lutein epoxide and β-cryptoxanthin) and three hydrocarbon carotenes (α-carotene, β-carotene and lycopene) was achieved using high-performance liquid chromatography with a reverse-phase C-30 column. Total carotenoid content declined up 71% through the digestion process. In vitro bioaccessibility of carotenoids was significantly higher in dairy products formulated with whole milk than those with skimmed milk, representing a difference of more than 21% (in the formulation using persimmon whole fruit as ingredient). Furthermore, addition of whole milk to any type of persimmon tissue significantly improved the bioaccessibility of total provitamin A carotenoids, reaching the highest values (38%) with whole fruit and whole milk.
The higher fat content in whole milk exerted a significant influence on carotenoid bioaccessibility, especially when using freeze-dried persimmon whole fruit. © 2017 Society of Chemical Industry.
在乳制品中添加富含类胡萝卜素的柿子,是获得功能性食品的一种替代方法。然而,类胡萝卜素的生物可及性受到脂肪含量和食物成分的强烈影响。这就是为什么要在含有全脂(3.6%脂肪)或脱脂牛奶(0.25%脂肪)和不同冻干柿组织的柿基乳制品中评估个体类胡萝卜素的体外生物可及性。
使用高效液相色谱法(反相 C-30 柱),可以明确鉴定出七种叶黄素(新黄质、玉米黄质、花药黄质、叶黄素、玉米黄质、叶黄素环氧化物和β-隐黄质)和三种类胡萝卜素(α-胡萝卜素、β-胡萝卜素和番茄红素)。通过消化过程,总类胡萝卜素含量下降了 71%。与脱脂牛奶相比,全脂牛奶配方的乳制品中类胡萝卜素的体外生物可及性显著提高,差异超过 21%(在使用柿全果作为配料的配方中)。此外,向任何类型的柿组织中添加全脂牛奶均可显著提高总维生素 A 类胡萝卜素的生物可及性,其中全果和全脂牛奶的生物可及性最高(38%)。
全脂牛奶中的较高脂肪含量对类胡萝卜素的生物可及性有显著影响,特别是当使用冻干柿全果时。 © 2017 化学工业协会。