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通过超高效液相色谱与高分辨率质谱联用分析两种品种的酚类物质谱差异及抗氧化能力

Differences in the Phenolic Profile by UPLC Coupled to High Resolution Mass Spectrometry and Antioxidant Capacity of Two Varieties.

作者信息

Esteban-Muñoz Adelaida, Sánchez-Hernández Silvia, Samaniego-Sánchez Cristina, Giménez-Martínez Rafael, Olalla-Herrera Manuel

机构信息

Departamento de Nutrición y Bromatología, Universidad de Granada, 18071 Granada, Spain.

Programme in Nutrition and Food Science, University of Granada, 18071 Granada, Spain.

出版信息

Antioxidants (Basel). 2020 Dec 30;10(1):31. doi: 10.3390/antiox10010031.

Abstract

BACKGROUND

phenolic compounds are bioactive chemical species derived from fruits and vegetables, with a plethora of healthy properties. In recent years, there has been a growing interest in persimmon ( L.f.) due to the presence of many different classes of phenolic compounds. However, the analysis of individual phenolic compounds is difficult due to matrix interferences.

METHODS

the aim of this research was the evaluation of individual phenolic compounds and antioxidant capacity of the pulp of two varieties of persimmon ( and ) by an improved extraction procedure together with a UPLC-Q-TOF-MS platform.

RESULTS

the phenolic compounds composition of persimmon was characterized by the presence of hydroxybenzoic and hydroxycinnamic acids, hydroxybenzaldehydes, dihydrochalcones, tyrosols, flavanols, flavanones, and flavonols. A total of 31 compounds were identified and 17 compounds were quantified. Gallic acid was the predominant phenolic compounds found in the variety (0.953 mg/100 g) whereas the concentration of -hydroxybenzoic acid was higher in the option (0.119 mg/100 g).

CONCLUSIONS

the results showed that the variety had higher quantities of phenolic compounds than the example. These data could be used as reference in future phenolic compound databases when individual health effects of phenolic compounds become available.

摘要

背景

酚类化合物是源自水果和蔬菜的生物活性化学物质,具有众多健康特性。近年来,由于柿子(柿属)中存在许多不同种类的酚类化合物,人们对其兴趣日益浓厚。然而,由于基质干扰,对单个酚类化合物的分析较为困难。

方法

本研究的目的是通过改进的提取方法以及超高效液相色谱-四极杆-飞行时间质谱平台,评估两种柿子品种( 和 )果肉中单个酚类化合物及其抗氧化能力。

结果

柿子的酚类化合物组成特点是含有羟基苯甲酸、羟基肉桂酸、羟基苯甲醛、二氢查耳酮、酪醇、黄烷醇、黄烷酮和黄酮醇。共鉴定出31种化合物,定量了17种化合物。没食子酸是 品种中含量最高的酚类化合物(0.953毫克/100克),而 品种中对羟基苯甲酸的浓度更高(0.119毫克/100克)。

结论

结果表明, 品种的酚类化合物含量高于 品种。当酚类化合物的个体健康效应明确时,这些数据可作为未来酚类化合物数据库的参考。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7955/7823481/c93ef990d471/antioxidants-10-00031-g001.jpg

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