Chu Y, Han G X, Wang Y Q, Shan H Y, Chen X P, Tao L Y, Zhang M Y
Department of Forensic Medicine, Medical College of Soochow University, Suzhou 215123, China.
Ying- kou Public Security Bureau, Yingkou 115000, China.
Fa Yi Xue Za Zhi. 2017 Jun;33(3):221-224. doi: 10.3969/j.issn.1004-5619.2017.03.001. Epub 2017 Jun 25.
To observe the changes of cystathionine β-synthase （CBS） expression in the cerebral cortex after brain contusion at different times.
An experimental model of traumatic brain injury （TBI） in mice was established by an improved weight-drop device. Then Western blotting and immunohistochemical examination were used to detect the CBS expression in cerebral cortex around injury at different time points （1 h, 6 h, 12 h, 1 d, 2 d, 3 d, 7 d）.
The results of Western blotting revealed that the expression level of CBS was down-regulated and reached its lowest level at the 3rd days after injury, and then restored to normal level after 7 days. The results of immunohistochemistry showed that CBS was present in the normal brain cortex. CBS expression gradually decreased at the 3rd days after injury, and then restored to normal level after 7 days.
CBS has the potential to be a reference index for time estimation after brain contusion in forensic practice.
观察脑挫伤后不同时间点大脑皮质中胱硫醚β-合酶（CBS）表达的变化。
采用改良的自由落体装置建立小鼠创伤性脑损伤（TBI）实验模型。然后运用蛋白质免疫印迹法和免疫组织化学检测不同时间点（1小时、6小时、12小时、1天、2天、3天、7天）损伤周围大脑皮质中CBS的表达。
蛋白质免疫印迹法结果显示，CBS的表达水平下调，在损伤后第3天达到最低水平，然后在7天后恢复到正常水平。免疫组织化学结果表明，CBS存在于正常脑皮质中。损伤后第3天CBS表达逐渐降低，然后在7天后恢复到正常水平。
在法医学实践中，CBS有可能成为脑挫伤后时间推断的参考指标。
