Auwal Shehu Muhammad, Zarei Mohammad, Abdul-Hamid Azizah, Saari Nazamid
Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
Department of Biochemistry, Faculty of Basic Medical Sciences, Bayero University, Kano, Nigeria.
Evid Based Complement Alternat Med. 2017;2017:4765463. doi: 10.1155/2017/4765463. Epub 2017 Oct 4.
Protein hydrolysates produced from different food sources exhibit therapeutic potential and can be used in the management of chronic diseases. This study was targeted to optimise the conditions for the hydrolysis of stone fish protein to produce antioxidant hydrolysates using central composite design (CCD) by response surface methodology (RSM). The stone fish protein was hydrolysed under the optimum predicted conditions defined by pH (6.5), temperature (54°C), E/S ratio (1.5%), and hydrolysis time (360 min). The hydrolysates were then evaluated for 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) scavenging activity and ferrous ion- (Fe-) chelating activity. Results validation showed no significant difference between the experimental values of DPPH scavenging activity (48.94%) and Fe-chelating activity (25.12%) obtained at 54.62% degree of hydrolysis (DH) compared to their corresponding predicted values of 49.79% and 24.08% at 53.08% DH, respectively. The hydrolysates demonstrated non-Newtonian behavior ( < 1) with stronger shear-thinning effect and higher viscosities at increasing concentration. Thus, RSM can be considered as a promising strategy to optimise the production of stone fish protein hydrolysates containing antioxidant peptides. It is hoped that this finding will enhance the potential of stone fish protein hydrolysates (SHs) as therapeutic bioactive ingredient in functional foods development.
不同食物来源产生的蛋白质水解物具有治疗潜力，可用于慢性病的管理。本研究旨在采用响应面法（RSM）通过中心复合设计（CCD）优化石鱼蛋白水解条件以生产抗氧化水解物。在由pH（6.5）、温度（54°C）、酶与底物比例（E/S比，1.5%）和水解时间（360分钟）确定的最佳预测条件下对石鱼蛋白进行水解。然后评估水解物的2,2-二苯基-1-苦基肼自由基（DPPH）清除活性和亚铁离子（Fe²⁺）螯合活性。结果验证表明，在水解度（DH）为54.62%时获得的DPPH清除活性实验值（48.94%）和Fe²⁺螯合活性实验值（25.12%）与在DH为53.08%时其相应预测值49.79%和24.08%之间无显著差异。水解物表现出非牛顿行为（n<1），具有更强的剪切变稀效应，且浓度增加时粘度更高。因此，RSM可被视为优化含抗氧化肽的石鱼蛋白水解物生产的一种有前景的策略。希望这一发现将提高石鱼蛋白水解物（SHs）作为功能性食品开发中治疗性生物活性成分的潜力。
