Chalamaiah M, Jyothirmayi T, Diwan Prakash V, Dinesh Kumar B
National Institute of Nutrition (Indian Council of Medical Research), Tarnaka, Hyderabad, 500 007 India.
Resource Centre, Central Food Technological Research Institute (Council of Scientific and Industrial Research), Hubshiguda, Uppal Road, Hyderabad, 500 007 India.
J Food Sci Technol. 2015 Sep;52(9):5817-25. doi: 10.1007/s13197-015-1714-6. Epub 2015 Jan 14.
Previously, we have reported the composition, molecular mass distribution and in vivo immunomodulatory effects of common carp roe protein hydrolysates. In the current study, antioxidative activity and functional properties of common carp (Cyprinus carpio) roe (egg) protein hydrolysates, prepared by pepsin, trypsin and Alcalase, were evaluated. The three hydrolysates showed excellent antioxidant activities in a dose dependent manner in various in vitro models such as 2,2 diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, 2,2'-azino-bis(3-ethylbenzthiazoline-6)-sulfonic acid (ABTS(+)) radical scavenging activity, ferric reducing antioxidant power (FRAP) and ferrous ion (Fe(2+)) chelating ability. Enzymatic hydrolysis significantly increased protein solubility of the hydrolysates to above 62 % over a wide pH range (2-12). Carp roe hydrolysates exhibited good foaming and emulsification properties. The results suggest that bioactive carp roe protein hydrolysates (CRPHs) with good functional properties could be useful in health food/nutraceutical/pharmaceutical industry for various applications.
此前，我们已报道了鲤鱼鱼籽蛋白水解物的组成、分子量分布及体内免疫调节作用。在本研究中，对通过胃蛋白酶、胰蛋白酶和碱性蛋白酶制备的鲤鱼（Cyprinus carpio）鱼籽（卵）蛋白水解物的抗氧化活性和功能特性进行了评估。这三种水解物在多种体外模型中，如2,2-二苯基-1-苦基肼（DPPH）自由基清除活性、2,2'-联氮-双-（3-乙基苯并噻唑啉-6-磺酸）（ABTS(+)）自由基清除活性、铁还原抗氧化能力（FRAP）和亚铁离子（Fe(2+)）螯合能力，均呈现出剂量依赖性的优异抗氧化活性。酶解显著提高了水解物在较宽pH范围（2 - 12）内的蛋白溶解度，使其超过62%。鲤鱼鱼籽水解物表现出良好的发泡和乳化性能。结果表明，具有良好功能特性的生物活性鲤鱼鱼籽蛋白水解物（CRPHs）在保健食品/营养保健品/制药行业的各种应用中可能具有实用价值。
