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加味芍药甘草汤及其含药血清对人子宫腺肌病病灶细胞增殖、凋亡及超微结构的影响

Effects of Jiawei Shaoyao-Gancao Decoction and Its Drug-Containing Serum on Proliferation, Apoptosis, and Ultrastructure of Human Adenomyosis Foci Cells.

作者信息

Zeng Yu-Yan, Li Kun-Yin

机构信息

Department of Gynecology, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510120, China.

Postdoctoral Programme, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.

出版信息

Evid Based Complement Alternat Med. 2017;2017:7821095. doi: 10.1155/2017/7821095. Epub 2017 Sep 12.

DOI:10.1155/2017/7821095
PMID:29234429
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5613445/
Abstract

OBJECTIVE

The present study aimed to investigate the effects of Jiawei Shaoyao-Gancao Decoction (JSGD) and its drug-containing serum (CDS) on the proliferation, apoptosis, and ultrastructure of human adenomyosis foci cells.

METHODS

Primary cultures of human adenomyosis foci cells were prepared from hard uterine lesions of adenomyosis patients. The cells were treated with JSGD (10 and 20 mg/ml), CDS, and mifepristone (MIF) for 24 or 48 h. Cell proliferation was detected using CCK-8 assay, cell apoptosis was measured by flow cytometry, and the cell ultrastructure was observed by transmission electron microscopy (TEM).

RESULTS

JSGD and CDS significantly induced cell apoptosis and inhibited cell proliferation for 24 h or 48 h, in which the effects of JSGD were in a dose-dependent manner. The effect of CDS for 24 h was higher than that of CDS for 48 h. Moreover, JSGD and CDS treatments induced marked apoptosis in adenomyosis foci cells, characterized by nucleus chromatin, condensation, fragmentation, mitochondria and endoplasmic swelling, and autophagy-lysosome.

CONCLUSIONS

JSGD and CDS can suppress proliferation and induce apoptosis in adenomyosis foci cells, through altering their ultrastructure. The results provided support for JSGD and CDS in the treatment of adenomyosis and gained further insight into the effect of this prescription.

摘要

目的

本研究旨在探讨加味芍药甘草汤（JSGD）及其含药血清（CDS）对人子宫腺肌病病灶细胞增殖、凋亡及超微结构的影响。

方法

从子宫腺肌病患者的子宫硬病灶中制备人子宫腺肌病病灶细胞原代培养物。将细胞用JSGD（10和20mg/ml）、CDS和米非司酮（MIF）处理24或48小时。使用CCK-8法检测细胞增殖，通过流式细胞术检测细胞凋亡，并通过透射电子显微镜（TEM）观察细胞超微结构。

结果

JSGD和CDS在24小时或48小时时均显著诱导细胞凋亡并抑制细胞增殖，其中JSGD的作用呈剂量依赖性。CDS处理24小时的效果高于处理48小时的效果。此外，JSGD和CDS处理诱导子宫腺肌病病灶细胞显著凋亡，其特征为细胞核染色质浓缩、碎片化、线粒体和内质网肿胀以及自噬溶酶体形成。

结论

JSGD和CDS可通过改变子宫腺肌病病灶细胞的超微结构来抑制其增殖并诱导凋亡。该结果为JSGD和CDS治疗子宫腺肌病提供了支持，并进一步深入了解了该方剂的作用效果。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b32b/5613445/905862bfb3c9/ECAM2017-7821095.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b32b/5613445/81dedba5a9fb/ECAM2017-7821095.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b32b/5613445/116065d78122/ECAM2017-7821095.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b32b/5613445/bd7db4f4ec7d/ECAM2017-7821095.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b32b/5613445/905862bfb3c9/ECAM2017-7821095.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b32b/5613445/81dedba5a9fb/ECAM2017-7821095.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b32b/5613445/116065d78122/ECAM2017-7821095.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b32b/5613445/bd7db4f4ec7d/ECAM2017-7821095.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b32b/5613445/905862bfb3c9/ECAM2017-7821095.004.jpg

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