Department of Polymer Science, University of Akron , Akron, Ohio 44325, United States.
Department of Chemistry, University of Akron , Akron, Ohio 44325, United States.
J Am Chem Soc. 2018 Jan 10;140(1):277-284. doi: 10.1021/jacs.7b09978. Epub 2017 Dec 27.
The ring-opening copolymerization of maleic anhydride and propylene oxide, using a functionalized primary alcohol initiator and magnesium 2,6-di-tert-butyl phenoxide as a catalyst, was investigated in order to produce high end-group fidelity poly(propylene maleate). Subsequent isomerization of the material into 3D printable poly(propylene fumarate) was utilized to produce thin films and scaffolds possessing groups that can be modified with bioactive groups postpolymerization and postprinting. The surface concentration of these modifiable groups was determined to be 30.0 ± 3.3 pmol·cm, and copper-mediated azide-alkyne cycloaddition was used to attach a small molecule dye and cell adhesive GRGDS peptides to the surface as a model system. The films were then studied for cytotoxicity and found to have high cell viability before and after surface modification.
马来酸酐和环氧丙烷的开环共聚反应，使用功能化的伯醇引发剂和镁 2,6-二叔丁基苯酚盐作为催化剂，以生产具有高端基保真度的聚（马来酸丙烯酯）。随后将该材料异构化为可 3D 打印的聚（富马酸丙烯酯），用于生产具有可在聚合后和打印后进行生物活性基团修饰的基团的薄膜和支架。这些可修饰基团的表面浓度被确定为 30.0 ± 3.3 pmol·cm，并且使用铜介导的叠氮-炔环加成反应将小分子染料和细胞黏附肽 GRGDS 连接到表面上作为模型系统。然后研究了这些薄膜的细胞毒性，发现表面修饰前后的细胞存活率都很高。
