a Department of Molecular Medicine, College of Medicine , Gachon University , Incheon , Korea.
b Soonchunhyang Institute of Medi-bio Science , Soonchunhyang University , Cheonan-si , Republic of Korea.
J Biomater Sci Polym Ed. 2018 May-Jun;29(7-9):917-931. doi: 10.1080/09205063.2017.1383020. Epub 2017 Sep 29.
Poly(propylene fumarate) (PPF) has known to be a good candidate material for cartilage tissue regeneration because of its excellent mechanical properties during its degradation processes. Here, we describe the potential application of PPF-based materials as 3D printing bioinks to create macroporous cell scaffolds using micro-stereolithography. To improve cell-matrix interaction of seeded human chondrocytes within the PPF-based 3D scaffolds, we immobilized arginine-glycine-aspartate (RGD) peptide onto the PPF scaffolds. We also evaluated various cellular behaviors of the seeded chondrocytes using MTS assay, microscopic and histological analyses. The results indicated that PPF-based biocompatible scaffolds with immobilized RGD peptide could effectively support initial adhesion and proliferation of human chondrocytes. Such a 3D bio-printable scaffold can offer an opportunity to promote cartilage tissue regeneration.
聚（富马酸丙二醇酯）（PPF）由于其在降解过程中的优异机械性能，已被证明是软骨组织再生的良好候选材料。在这里，我们描述了基于 PPF 的材料作为 3D 打印生物墨水的潜在应用，使用微立体光刻技术创建大孔细胞支架。为了提高 PPF 基 3D 支架中接种的人软骨细胞与细胞外基质的相互作用，我们将精氨酸-甘氨酸-天冬氨酸（RGD）肽固定在 PPF 支架上。我们还使用 MTS 测定法、显微镜和组织学分析评估了接种的软骨细胞的各种细胞行为。结果表明，固定有 RGD 肽的基于 PPF 的生物相容性支架可以有效地支持人软骨细胞的初始黏附和增殖。这种 3D 可生物打印支架为促进软骨组织再生提供了机会。
