Loong Shih Keng, Che-Mat-Seri Nurul-Asma-Anati, Abdulrazak Osama, Douadi Benacer, Ahmad-Nasrah Siti-Noraisah, Johari Jefree, Mohd-Zain Siti-Nursheena, Abubakar Sazaly
Tropical Infectious Diseases Research & Education Centre, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Microbial Evolutionary Dynamics Research Group, Department of Evolutionary Theory, Max Planck Institute for Evolutionary Biology, 24306 Plön, Germany.
J Vet Med Sci. 2018 Jan 27;80(1):77-84. doi: 10.1292/jvms.17-0218. Epub 2017 Dec 13.
Rodents have historically been associated with zoonotic pandemics that claimed the lives of large human populations. Appropriate pathogen surveillance initiatives could contribute to early detection of zoonotic infections to prevent future outbreaks. Bordetella species are bacteria known to cause mild to severe respiratory disease in mammals and, some have been described to infect, colonize and spread in rodents. There is a lack of information on the population diversity of bordetellae among Malaysian wild rodents. Here, bordetellae recovered from lung tissues of wild rats were genotypically characterized using 16S rDNA sequencing, MLST and nrdA typing. A novel B. bronchiseptica ST82, closely related to other human-derived isolates, was discovered in three wild rats (n=3) from Terengganu (5.3333° N, 103.1500° E). B. pseudohinzii, a recently identified laboratory mice inhabitant, was also recovered from one rat (n=1). Both bordetellae displayed identical antimicrobial resistance profiles, indicating the close phylogenetic association between them. Genotyping using the 765-bp nrdA locus was shown to be compatible with the MLST-based phylogeny, with the added advantage of being able to genotype non-classical bordetellae. The recovery of B. pseudohinzii from wild rat implied that this bordetellae has a wider host range than previously thought. The findings from this study suggest that bordetellae surveillance among wild rats in Malaysia has to be continued and expanded to other states to ensure early identification of species capable of causing public health disorder.
从历史上看，啮齿动物一直与导致大量人类死亡的人畜共患大流行有关。适当的病原体监测举措有助于早期发现人畜共患感染，以预防未来的疫情爆发。博德特氏菌属细菌已知会在哺乳动物中引起轻度至重度呼吸道疾病，并且，一些已被描述为可在啮齿动物中感染、定殖和传播。关于马来西亚野生啮齿动物中博德特氏菌属的种群多样性缺乏相关信息。在此，对从野生大鼠肺组织中分离出的博德特氏菌属进行了基因分型，采用了16S rDNA测序、多位点序列分型（MLST）和nrdA分型。在来自登嘉楼（北纬5.3333°，东经103.1500°）的三只野生大鼠（n = 3）中发现了一种新型支气管败血博德特氏菌ST82，它与其他源自人类的分离株密切相关。伪欣氏博德特氏菌是最近在实验室小鼠中发现的一种菌，也从一只大鼠（n = 1）中分离出来。这两种博德特氏菌显示出相同的抗菌耐药谱，表明它们之间存在密切的系统发育关联。使用765bp的nrdA基因座进行基因分型与基于MLST的系统发育分析兼容，其额外优势在于能够对非典型博德特氏菌进行基因分型。从野生大鼠中分离出伪欣氏博德特氏菌意味着这种博德特氏菌的宿主范围比以前认为的更广。本研究结果表明，马来西亚必须继续并扩大对野生大鼠中博德特氏菌属的监测，以确保早期识别能够引起公共卫生疾病的菌种。
