Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian, People's Republic of China.
Epidemiol Infect. 2020 Aug 24;148:e237. doi: 10.1017/S0950268820001879.
Bordetella bronchiseptica is a potential zoonotic pathogen, which mainly causes respiratory diseases in humans and a variety of animal species. B. bronchiseptica is one of the important pathogens isolated from rabbits in Fujian Province. However, the knowledge of the epidemiology and characteristics of the B. bronchiseptica in rabbits in Fujian Province is largely unknown. In this study, 219 B. bronchiseptica isolates recovered from lung samples of dead rabbits with respiratory diseases in Fujian Province were characterised by multi-locus sequencing typing, screening virulence genes and testing antimicrobial susceptibility. The results showed that the 219 isolates were typed into 11 sequence types (STs) including five known STs (ST6, ST10, ST12, ST14 and ST33) and six new STs (ST88, ST89, ST90, ST91, ST92 and ST93) and the ST33 (30.14%, 66/219), ST14 (26.94%, 59/219) and ST12 (16.44%, 36/219) were the three most prevalent STs. Surprisingly, all the 219 isolates carried the five virulence genes (fhaB, prn, cyaA, dnt and bteA) in the polymerase chain reaction screening. Moreover, the isolates were resistant to cefixime, ceftizoxime, cefatriaxone and ampicillin at rates of 33.33%, 31.05%, 11.87% and 3.20%, respectively. This study showed the genetic diversity of B. bronchiseptica in rabbits in Fujian Province, and the colonisation of the human-associated ST12 strain in rabbits in Fujian Province. The results might be useful for monitoring the epidemic strains, developing preventive methods and preventing the transmission of epidemic strains from rabbits to humans.
支气管败血波氏杆菌是一种潜在的人畜共患病病原体，主要引起人类和多种动物的呼吸道疾病。支气管败血波氏杆菌是从福建省兔中分离到的重要病原体之一。然而，福建省兔中支气管败血波氏杆菌的流行病学和特征知识在很大程度上尚不清楚。在本研究中，从福建省患有呼吸道疾病的病死兔的肺组织样本中分离出 219 株支气管败血波氏杆菌，通过多位点序列分型、筛选毒力基因和检测抗菌药物敏感性对其进行了特征分析。结果表明，219 株分离株被分为 11 个序列型（ST），包括 5 个已知 ST（ST6、ST10、ST12、ST14 和 ST33）和 6 个新 ST（ST88、ST89、ST90、ST91、ST92 和 ST93），其中 ST33（30.14%，66/219）、ST14（26.94%，59/219）和 ST12（16.44%，36/219）是最常见的 3 个 ST。令人惊讶的是，所有 219 株分离株在聚合酶链反应筛选中均携带 5 个毒力基因（fhaB、prn、cyaA、dnt 和 bteA）。此外，分离株对头孢克肟、头孢噻肟、头孢曲松和氨苄西林的耐药率分别为 33.33%、31.05%、11.87%和 3.20%。本研究显示了福建省兔中支气管败血波氏杆菌的遗传多样性，以及人类相关 ST12 菌株在福建省兔中的定植情况。研究结果可能有助于监测流行菌株，开发预防方法，并防止流行菌株从兔传播给人类。
