Wang Ye, Pawlowski Michal E, Tkaczyk Tomasz S
Rice University, Department of Bioengineering, Houston, Texas, United States.
Opt Eng. 2017;56(8). doi: 10.1117/1.OE.56.8.081803. Epub 2017 May 2.
A prototype fiber-based imaging spectrometer was developed to provide snapshot hyperspectral imaging tuned for biomedical applications. The system is designed for imaging in the visible spectral range from 400 to 700 nm for compatibility with molecular imaging applications as well as satellite and remote sensing. An 81 × 96 pixel spatial sampling density is achieved by using a custom-made fiber-optic bundle. The design considerations and fabrication aspects of the fiber bundle and imaging spectrometer are described in detail. Through the custom fiber bundle, the image of a scene of interest is collected and divided into discrete spatial groups, with spaces generated in between groups for spectral dispersion. This reorganized image is scaled down by an image taper for compatibility with following optical elements, dispersed by a prism, and is finally acquired by a CCD camera. To obtain an () datacube from the snapshot measurement, a spectral calibration algorithm is executed for reconstruction of the spatial-spectral signatures of the observed scene. System characterization of throughput, resolution, and crosstalk was performed. Preliminary results illustrating changes in oxygen-saturation in an occluded human finger are presented to demonstrate the system's capabilities.
开发了一种基于光纤的成像光谱仪原型，以提供针对生物医学应用进行调谐的快照高光谱成像。该系统设计用于在400至700纳米的可见光谱范围内成像，以便与分子成像应用以及卫星和遥感兼容。通过使用定制的光纤束实现了81×96像素的空间采样密度。详细描述了光纤束和成像光谱仪的设计考虑因素和制造方面。通过定制光纤束，收集感兴趣场景的图像并将其划分为离散的空间组，在组之间生成空间用于光谱色散。该重组图像通过图像锥度缩小以与后续光学元件兼容，由棱镜色散，最后由电荷耦合器件（CCD）相机采集。为了从快照测量中获得（）数据立方体，执行光谱校准算法以重建观察场景的空间光谱特征。对系统的通量、分辨率和串扰进行了表征。展示了说明被阻塞的人手指中氧饱和度变化的初步结果，以证明该系统的能力。
