解脂耶氏酵母酵母型中可去阻遏酸性磷酸酶的动力学特性
Kinetic properties of derepressible acid phosphatase from the yeast from of Yarrowia lipolytica.
作者信息
Moran A, Burguillo F J, López M C, Domínguez A
机构信息
Departamento de Química Fisica, Facultad de Farmacia, Universidad de Salamanca, Spain.
出版信息
Biochim Biophys Acta. 1989 Mar 24;990(3):288-96. doi: 10.1016/s0304-4165(89)80047-9.
(1) An acid phosphatase from depressed cells of the yeast form of Yarrowia lipolytica has been characterized kinetically by studies on specificity, inhibition, rate equation forms and modelling of the enzyme mechanisms. (2) The study on specificity revealed that the acid phosphatase is a rather unspecific phosphohydrolase that has similar activity on several different phosphate esters. A very weak transphosphorylating activity was also detected. (3) Among the reversible inhibitors, phosphate and vanadate were outstanding, whereas EDTA behaved as an activator. (4) v vs. [S] studies with o-carboxyphenyl phosphate as substrate show that the acid phosphatase of Y. lipolytica exhibits non-Michaelian behaviour, a minimum degree of 2:2 being detected for the rate equation in [S]. (5) The inhibition by phosphate and vanadate seems to have the same pattern of partial inhibition with a certain non-competitive nature, 1:1 being the minimum degree of the rate equation detected in (I).
(1) 通过对解脂耶氏酵母酵母形式的抑郁细胞中的酸性磷酸酶进行特异性、抑制作用、速率方程形式和酶机制建模等研究，对其进行了动力学表征。(2) 特异性研究表明，该酸性磷酸酶是一种相当非特异性的磷酸水解酶，对几种不同的磷酸酯具有相似的活性。还检测到非常微弱的转磷酸化活性。(3) 在可逆抑制剂中，磷酸盐和钒酸盐表现突出，而乙二胺四乙酸（EDTA）表现为激活剂。(4) 以邻羧基苯磷酸为底物进行的v对[S]研究表明，解脂耶氏酵母的酸性磷酸酶表现出非米氏行为，在[S]中的速率方程检测到的最小程度为2:2。(5) 磷酸盐和钒酸盐的抑制作用似乎具有相同的部分抑制模式，具有一定的非竞争性性质，在(I)中检测到的速率方程的最小程度为1:1。
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