生长温度对解脂耶氏酵母酸性磷酸酶活性的影响。

Influence of growth temperature on the acid phosphatase activity in the yeast Yarrowia lipolytica.

作者信息

Vasileva-Tonkova E, Balasheva D M, Galabova D

机构信息

Department of Microbial Biochemistry and Biosynthesis, Bulgarian Academy of Sciences, Acad. G. Bonchev str., Sofia, Bulgaria.

出版信息

FEMS Microbiol Lett. 1996 Dec 1;145(2):267-71. doi: 10.1111/j.1574-6968.1996.tb08588.x.

DOI:10.1111/j.1574-6968.1996.tb08588.x

PMID:8961566

Abstract

The adaptation of the yeast Yarrowia lipolytica 76-18 to growth temperature was studied by measuring the levels of secreted and intracellular acid phosphatase activities during growth at five temperatures from 8 to 36 degrees C. The intracellular acid phosphatase activity is maximal at a growth temperature of 20 degrees C. The level of the secreted phosphatase activity decreases as growth temperature increases from 15 to 36 degrees C. It is the growth temperature itself and not the growth rate that regulates these activities. The observed dependence of the acid phosphatase activity on the growth temperature indicates a possible participation of acid phosphatases in the temperature adaptation of yeast cells.

摘要

通过测量解脂耶氏酵母76-18在8至36摄氏度五个温度下生长期间分泌型和细胞内酸性磷酸酶活性水平，研究了该酵母对生长温度的适应性。细胞内酸性磷酸酶活性在20摄氏度的生长温度下最高。随着生长温度从15摄氏度升高到36摄氏度，分泌型磷酸酶活性水平降低。调节这些活性的是生长温度本身而非生长速率。观察到的酸性磷酸酶活性对生长温度的依赖性表明酸性磷酸酶可能参与酵母细胞的温度适应过程。