Zou Yu-Qiong, Jin Wei-Dong, Li Ya-Song
1 Department of Rheumatology, Zhejiang Provincial People's Hospital, Hangzhou, Zhejiang, China.
2 Department of Clinical Laboratory, Zhejiang Provincial People's Hospital, Hangzhou, Zhejiang, China.
J Int Med Res. 2018 Feb;46(2):732-738. doi: 10.1177/0300060517726194. Epub 2017 Sep 14.
Objective To elucidate the clinical significance of macrophage migration inhibitory factor (MIF) serum concentration in patients with polymyositis. Methods Thirty-six patients with polymyositis were enrolled. Serum samples were obtained and stored to detect MIF and interleukin (IL)-6 using commercially available enzyme-linked immunosorbent assay kits. The relationships between these cytokines and clinical data were analyzed. Results The serum MIF concentration was significantly lower in patients in remission (34.74 ± 17.75) and in healthy controls (38.87 ± 9.30 ng/ml) than that in patients with active polymyositis (50.04 ± 23.84 ng/ml). There were no significant differences between healthy controls and patients in remission. The serum IL-6 concentration in patients with active polymyositis (19.67 ± 7.16 pg/ml) was significantly higher than that in patients in remission (15.81 ± 4.00 pg/ml) and controls (8.14 ± 3.71 pg/ml). The serum IL-6 concentration was negatively correlated with the serum MIF concentration (r = -0.283). No relationship was found between the serum MIF concentration and glucocorticoid dose. The MIF concentration peaked twice during treatment when the creatine kinase concentration was decreasing. Conclusion MIF and IL-6 play important roles in the inflammation associated with polymyositis. MIF might also be involved in the early stage of regeneration in polymyositis. MIF may thus serve as a biomarker of disease activity and outcome.
目的 阐明巨噬细胞移动抑制因子（MIF）血清浓度在多发性肌炎患者中的临床意义。方法 纳入36例多发性肌炎患者。采集血清样本并保存，使用市售酶联免疫吸附测定试剂盒检测MIF和白细胞介素（IL）-6。分析这些细胞因子与临床数据之间的关系。结果 缓解期患者（34.74±17.75）和健康对照者（38.87±9.30 ng/ml）的血清MIF浓度显著低于活动期多发性肌炎患者（50.04±23.84 ng/ml）。健康对照者与缓解期患者之间无显著差异。活动期多发性肌炎患者的血清IL-6浓度（19.67±7.16 pg/ml）显著高于缓解期患者（15.81±4.00 pg/ml）和对照者（8.14±3.71 pg/ml）。血清IL-6浓度与血清MIF浓度呈负相关（r = -0.283）。未发现血清MIF浓度与糖皮质激素剂量之间存在关联。在治疗过程中，当肌酸激酶浓度下降时，MIF浓度出现两次峰值。结论 MIF和IL-6在与多发性肌炎相关的炎症中起重要作用。MIF可能也参与了多发性肌炎的早期再生阶段。因此，MIF可作为疾病活动和预后的生物标志物。
