Rodrigues Rodrigo, Franke Rodrigo de Azevedo, Teixeira Bruno Costa, Macedo Rodrigo Cauduro de Oliveira, Lopes André Luiz, Reischak-Oliveira Álvaro, Baroni Bruno Manfredini, Vaz Marco Aurélio
Universidade Federal do Rio Grande do Sul, Felizardo St, 750 – Porto Alegre, Rio Grande do Sul, 90690-200, Brazil.
Centro Universitário da Serra Gaúcha, Caxias do Sul, Os Dezoito do Forte St, 2366 – Caxias do Sul, Rio Grande do Sul, 95020-472, RS, Brazil.
Chin J Physiol. 2017 Dec 31;60(6):327-337. doi: 10.4077/CJP.2017.BAG519.
Alcohol (ALC) intake and sleep deprivation (SDP) are conditions that cause changes in the physiological status. However, the relationship between biological markers and mood states is not entirely clear yet. The purpose of the study was to compare isolated and combined effects of ALC intake and SDP on hormonal and inflammatory responses and in changes in the state of mood. Also, we intended to explain possible changes in mood states through biochemical variations using multiple stepwise regression analysis. Ten healthy male were randomized in four situations: (1) placebo intake + normal sleep (PLA + SLE); (2) ALC intake + SLE; (3) PLA intake + SDP; (4) ALC intake + SDP. While subjects ingested ALC (1 g/kg of beer), PLA intake was a non-alcoholic beer. The subjects had one night of SLE or were subjected to SDP in the lab for 8 h. After each experimental condition, morning blood samples were taken for assessments of serum levels of glucose, cortisol, testosterone, epinephrine, interleukin-6 (IL-6), interleukin-10 (IL-10) and C-reactive protein (CRP). The subjects were also asked to fill in a Profile of Mood State questionnaire. The results showed that the glucose level was significant lower in ALC + SDP compared to the PLA + SLE condition. Total Mood Disturbance was lower in ALC + SDP and PLA + SDP compared to the PLA + SLE condition. Fatigue was higher under SDP conditions compared to PLA + SLE. Vigor was lower under the ALC + SDP condition compared to the PLA + SLE condition. Regression analysis showed that Total Mood Disturbance and fatigue under ALC + SDP were associated with changes in the cortisol levels. Our results showed that combined and isolated ALC intake and one night of SDP did not change the hormonal and inflammatory responses tested, and the combined effects caused a reduction in the glucose levels. Vigor, fatigue and Total Mood Disturbance were affected by each condition. Furthermore, Total Mood Disturbance and fatigue were possibly explained by changes in the cortisol levels in the combined condition.
酒精（ALC）摄入和睡眠剥夺（SDP）是会导致生理状态发生变化的情况。然而，生物标志物与情绪状态之间的关系尚不完全清楚。本研究的目的是比较ALC摄入和SDP单独及联合作用对激素和炎症反应以及情绪状态变化的影响。此外，我们打算通过多元逐步回归分析，利用生化变化来解释情绪状态可能发生的变化。十名健康男性被随机分配到四种情况：（1）摄入安慰剂 + 正常睡眠（PLA + SLE）；（2）摄入ALC + SLE；（3）摄入PLA + SDP；（4）摄入ALC + SDP。当受试者摄入ALC（1克/千克啤酒）时，PLA摄入为无酒精啤酒。受试者有一晚的正常睡眠或在实验室接受8小时的睡眠剥夺。在每种实验条件后，采集早晨血样以评估血清葡萄糖、皮质醇、睾酮、肾上腺素、白细胞介素-6（IL-6）、白细胞介素-10（IL-10）和C反应蛋白（CRP）水平。受试者还被要求填写一份情绪状态剖面图问卷。结果显示，与PLA + SLE条件相比，ALC + SDP条件下的葡萄糖水平显著降低。与PLA + SLE条件相比，ALC + SDP和PLA + SDP条件下的总情绪紊乱程度较低。与PLA + SLE相比，睡眠剥夺条件下的疲劳程度更高。与PLA + SLE条件相比，ALC + SDP条件下的活力较低。回归分析表明，ALC + SDP条件下的总情绪紊乱和疲劳与皮质醇水平的变化有关。我们的结果表明，单独和联合的ALC摄入以及一晚的睡眠剥夺并未改变所测试的激素和炎症反应，联合作用导致葡萄糖水平降低。活力、疲劳和总情绪紊乱受到每种条件的影响。此外，在联合条件下，总情绪紊乱和疲劳可能由皮质醇水平的变化来解释。
