Department of Obstetrics, Universidade Federal de São Paulo, São Paulo, Brazil.
Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, Universidade de São Paulo, São Paulo, Brazil.
Cytokine. 2018 Jul;107:79-84. doi: 10.1016/j.cyto.2017.11.017. Epub 2017 Dec 11.
Gestational diabetes Mellitus has been considered an inflammatory disease involving different cells and mediators in its development. The role of innate immune cells in GDM physiopathology remains unclear, therefore this study was conducted to assess monocyte profile in GDM patients.
This was a case-control study including 20 glucose-tolerant pregnant women (controls) and 18 GDM patients.
Flow cytometry was used to assess peripheral blood monocytes subsets (classical, intermediate, non-classical), the expression of TLR4 and CCR2 chemokine receptor (CD192) and cytokines (TNFA, IL6, IL10) secretion by monocytes subsets. In addition, sCD14 serum levels were evaluated by ELISA.
We observed increased percentage of CD14 cells, decreased frequency of intermediate monocytes (CD14CD16), and lower percentage of circulating monocytes (classical, intermediate and non-classical) that express TLR4 in the diabetic group compared to controls. Soluble CD14 serum levels were higher in GDM patients compared to controls. There were no differences in the expression of the CCR2 chemokine receptor and cytokines (TNFA, IL6 and IL10) secretion between the studied groups.
Our results demonstrated that GDM patients present impaired monocyte profile in the peripheral blood, suggesting that these cells are involved in GDM physiopathology.
妊娠糖尿病被认为是一种涉及不同细胞和介质的炎症性疾病。先天免疫细胞在 GDM 病理生理学中的作用尚不清楚，因此本研究旨在评估 GDM 患者的单核细胞谱。
这是一项病例对照研究，包括 20 名糖耐量正常的孕妇（对照组）和 18 名 GDM 患者。
采用流式细胞术评估外周血单核细胞亚群（经典型、中间型、非经典型）、TLR4 和 CCR2 趋化因子受体（CD192）的表达以及单核细胞亚群分泌的细胞因子（TNFA、IL6、IL10）。此外，通过 ELISA 评估血清 sCD14 水平。
与对照组相比，糖尿病组 CD14 细胞百分比增加，中间型单核细胞（CD14CD16）频率降低，循环单核细胞（经典型、中间型和非经典型）的百分比降低，且 TLR4 表达降低。与对照组相比，GDM 患者的血清可溶性 CD14 水平更高。两组间 CCR2 趋化因子受体和细胞因子（TNFA、IL6 和 IL10）的表达无差异。
我们的研究结果表明，GDM 患者外周血单核细胞谱存在受损，提示这些细胞参与了 GDM 的病理生理学过程。
