Deys B F, Stap J
Br J Cancer. 1979 Nov;40(5):761-7. doi: 10.1038/bjc.1979.258.
Analysis of time-lapse cinematographic film permitted the construction of pedigrees from 88 well oxygenated cells of a mouse osteosarcoma (MOS). These cells have been chronically treated with various concentrations of the hypoxic cell sensitizer misonidazole (MIS) over periods of up to 96 h. At concentrations of 0.5 and 7 mM there is a 2--3 h increase in cell-cycle time. Concentrations of 2 mM show an intermitotic time delay of 7.6--10.3 h. At 4 mM cells divided only once. With increasing drug concentration there was an increase in the number of abnormal mitoses. These results were compared with cloning efficiency (PE) experiments. PE at 0.5 mM is 80%, at 1 mM 40 and at 2 mM is reduced to 4%. Cells treated with 2mM MIS over a period of 28.6 h resume their normal cycle when the drug is washed from the culture. This may indicate that DNA is not a major target for MIS. It is concluded that this hypoxic cell sensitizer is also toxic for MOS cells in well oxygenated conditions.
对延时摄影胶片的分析使得能够从一只小鼠骨肉瘤（MOS）的88个充分氧合的细胞构建谱系。这些细胞已经在长达96小时的时间里用不同浓度的低氧细胞增敏剂米索硝唑（MIS）进行了长期处理。在0.5和7 mM的浓度下，细胞周期时间增加了2 - 3小时。2 mM的浓度显示有丝分裂间期延迟7.6 - 10.3小时。在4 mM时细胞仅分裂一次。随着药物浓度增加，异常有丝分裂的数量增加。这些结果与克隆效率（PE）实验进行了比较。0.5 mM时的PE为80%，1 mM时为40%，2 mM时降至4%。用2 mM MIS处理28.6小时的细胞在药物从培养物中洗去后恢复其正常周期。这可能表明DNA不是MIS的主要靶点。得出的结论是，这种低氧细胞增敏剂在充分氧合的条件下对MOS细胞也有毒性。
