Filpa Viviana, Bistoletti Michela, Caon Ilaria, Moro Elisabetta, Grimaldi Annalisa, Moretto Paola, Baj Andreina, Giron Maria Cecilia, Karousou Evgenia, Viola Manuela, Crema Francesca, Frigo Gianmario, Passi Alberto, Giaroni Cristina, Vigetti Davide
Department of Medicine and Surgery, University of Insubria, via H. Dunant 5, Varese, Italy.
Department of Internal Medicine and Therapeutics, Section of Pharmacology, University of Pavia, Pavia, Italy.
Sci Rep. 2017 Dec 15;7(1):17644. doi: 10.1038/s41598-017-18020-7.
Myenteric plexus alterations hamper gastrointestinal motor function during intestinal inflammation. Hyaluronan (HA), an extracellular matrix glycosaminoglycan involved in inflammatory responses, may play a role in this process. In the colon of control rats, HA-binding protein (HABP), was detected in myenteric neuron soma, perineuronal space and ganglia surfaces. Prominent hyaluronan synthase 2 (HAS2) staining was found in myenteric neuron cytoplasm, suggesting that myenteric neurons produce HA. In the myenteric plexus of rats with 2, 4-dinitrobenzene sulfonic (DNBS)-induced colitis HABP staining was altered in the perineuronal space, while both HABP staining and HA levels increased in the muscularis propria. HAS2 immunopositive myenteric neurons and HAS2 mRNA and protein levels also increased. Overall, these observations suggest that inflammation alters HA distribution and levels in the gut neuromuscular compartment. Such changes may contribute to alterations in the myenteric plexus.
肌间神经丛改变会在肠道炎症期间妨碍胃肠运动功能。透明质酸（HA）是一种参与炎症反应的细胞外基质糖胺聚糖，可能在此过程中发挥作用。在对照大鼠的结肠中，在肌间神经节神经元胞体、神经周间隙和神经节表面检测到透明质酸结合蛋白（HABP）。在肌间神经节神经元细胞质中发现显著的透明质酸合酶2（HAS2）染色，表明肌间神经节神经元产生HA。在2,4-二硝基苯磺酸（DNBS）诱导的结肠炎大鼠的肌间神经丛中，神经周间隙的HABP染色发生改变，而固有肌层中的HABP染色和HA水平均升高。HAS2免疫阳性的肌间神经节神经元以及HAS2 mRNA和蛋白水平也升高。总体而言，这些观察结果表明炎症会改变肠道神经肌肉隔室中HA的分布和水平。此类变化可能导致肌间神经丛发生改变。
