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B染色体CAP - G假基因的转录不影响蝗虫中的正常凝聚素复合体基因。

Transcription of a B chromosome CAP-G pseudogene does not influence normal Condensin Complex genes in a grasshopper.

作者信息

Navarro-Domínguez Beatriz, Ruiz-Ruano Francisco J, Camacho Juan Pedro M, Cabrero Josefa, López-León María Dolores

机构信息

Departamento de Genética. Facultad de Ciencias, Universidad de Granada, 18071, Granada, Spain.

Department of Ecology, Evolution, and Organismal Biology, Iowa State University, Ames, Iowa, USA.

出版信息

Sci Rep. 2017 Dec 15;7(1):17650. doi: 10.1038/s41598-017-15894-5.

DOI:10.1038/s41598-017-15894-5
PMID:29247237
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5732253/
Abstract

Parasitic B chromosomes invade and persist in natural populations through several mechanisms for transmission advantage (drive). They may contain gene-derived sequences which, in some cases, are actively transcribed. A further interesting question is whether B-derived transcripts become functional products. In the grasshopper Eyprepocnemis plorans, one of the gene-derived sequences located on the B chromosome shows homology with the gene coding for the CAP-G subunit of condensin I. We show here, by means of fluorescent in situ hybridization coupled with tyramide signal amplification (FISH-TSA), that this gene is located in the distal region of the B24 chromosome variant. The DNA sequence located in the B chromosome is a pseudogenic version of the CAP-G gene (B-CAP-G). In two Spanish populations, we found active transcription of B-CAP-G, but it did not influence the expression of CAP-D2 and CAP-D3 genes coding for corresponding condensin I and II subunits, respectively. Our results indicate that the transcriptional regulation of the B-CAP-G pseudogene is uncoupled from the standard regulation of the genes that constitute the condensin complex, and suggest that some of the B chromosome known effects may be related with its gene content and transcriptional activity, thus opening new exciting avenues for research.

摘要

寄生B染色体通过几种具有传播优势（驱动）的机制侵入并在自然种群中持续存在。它们可能包含基因衍生序列，在某些情况下，这些序列会被积极转录。另一个有趣的问题是B染色体衍生的转录本是否会成为功能性产物。在蝗虫Eyprepocnemis plorans中，位于B染色体上的一个基因衍生序列与编码凝聚素I的CAP-G亚基的基因具有同源性。我们在此通过荧光原位杂交结合酪胺信号放大（FISH-TSA）表明，该基因位于B24染色体变体的远端区域。位于B染色体上的DNA序列是CAP-G基因（B-CAP-G）的假基因版本。在两个西班牙种群中，我们发现B-CAP-G有活跃转录，但它并未影响分别编码相应凝聚素I和II亚基的CAP-D2和CAP-D3基因的表达。我们的结果表明，B-CAP-G假基因的转录调控与构成凝聚素复合体的基因的标准调控是解耦的，并表明B染色体的一些已知效应可能与其基因含量和转录活性有关，从而为研究开辟了新的令人兴奋的途径。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce53/5732253/2b87074f88e1/41598_2017_15894_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce53/5732253/11576400b45c/41598_2017_15894_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce53/5732253/68dbc5454c2d/41598_2017_15894_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce53/5732253/0593d0a08cf6/41598_2017_15894_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce53/5732253/20d1f7b9bbd1/41598_2017_15894_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce53/5732253/78fa5d55c6dd/41598_2017_15894_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce53/5732253/a8fc6d3f21d3/41598_2017_15894_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce53/5732253/2b87074f88e1/41598_2017_15894_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce53/5732253/11576400b45c/41598_2017_15894_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce53/5732253/68dbc5454c2d/41598_2017_15894_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce53/5732253/0593d0a08cf6/41598_2017_15894_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce53/5732253/20d1f7b9bbd1/41598_2017_15894_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce53/5732253/78fa5d55c6dd/41598_2017_15894_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce53/5732253/a8fc6d3f21d3/41598_2017_15894_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce53/5732253/2b87074f88e1/41598_2017_15894_Fig7_HTML.jpg

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本文引用的文献

1
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Evolution. 1998 Feb;52(1):274-277. doi: 10.1111/j.1558-5646.1998.tb05163.x.
2
ORIGIN AND SUBSTITUTION OF B CHROMOSOMES IN THE GRASSHOPPER EYPREPOCNEMIS PLORANS.草蜢Eyprepocnemis plorans中B染色体的起源与替代
Evolution. 1990 May;44(3):747-753. doi: 10.1111/j.1558-5646.1990.tb05952.x.
3
Protein-coding genes in B chromosomes of the grasshopper Eyprepocnemis plorans.Eyprepocnemis plorans 染色体中的蛋白质编码基因。
卫星 DNA 含量在博茨瓦纳丽鱼科鱼类 Characidium gomesi 的 B 染色体中支持它们来自性染色体。
Mol Genet Genomics. 2020 Jan;295(1):195-207. doi: 10.1007/s00438-019-01615-2. Epub 2019 Oct 17.
4
Sequence Expression of Supernumerary B Chromosomes: Function or Fluff?额外 B 染色体的序列表达：功能还是空谈？
Genes (Basel). 2019 Feb 8;10(2):123. doi: 10.3390/genes10020123.
5
Gene expression changes elicited by a parasitic B chromosome in the grasshopper Eyprepocnemis plorans are consistent with its phenotypic effects.寄生B染色体在蝗虫Eyprepocnemis plorans中引发的基因表达变化与其表型效应一致。
Chromosoma. 2019 Mar;128(1):53-67. doi: 10.1007/s00412-018-00689-y. Epub 2019 Jan 7.
6
Genomic Characterization of a B Chromosome in Lake Malawi Cichlid Fishes.马拉维湖丽鱼科鱼类B染色体的基因组特征分析
Genes (Basel). 2018 Dec 5;9(12):610. doi: 10.3390/genes9120610.
7
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Gigascience. 2018 Dec 1;7(12):giy127. doi: 10.1093/gigascience/giy127.
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4
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5
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