Eyprepocnemis plorans 染色体中的蛋白质编码基因。
Protein-coding genes in B chromosomes of the grasshopper Eyprepocnemis plorans.
机构信息
Departamento de Genética, Facultad de Ciencias, Universidad de Granada, 18071 Granada, Spain.
Leibniz Institute for Plant Genetics and Crop Plant Research (IPK), D-06466 Gatersleben, Germany.
出版信息
Sci Rep. 2017 Apr 3;7:45200. doi: 10.1038/srep45200.
For many years, parasitic B chromosomes have been considered genetically inert elements. Here we show the presence of ten protein-coding genes in the B chromosome of the grasshopper Eyprepocnemis plorans. Four of these genes (CIP2A, GTPB6, KIF20A, and MTG1) were complete in the B chromosome whereas the six remaining (CKAP2, CAP-G, HYI, MYCB2, SLIT and TOP2A) were truncated. Five of these genes (CIP2A, CKAP2, CAP-G, KIF20A, and MYCB2) were significantly up-regulated in B-carrying individuals, as expected if they were actively transcribed from the B chromosome. This conclusion is supported by three truncated genes (CKAP2, CAP-G and MYCB2) which showed up-regulation only in the regions being present in the B chromosome. Our results indicate that B chromosomes are not so silenced as was hitherto believed. Interestingly, the five active genes in the B chromosome code for functions related with cell division, which is the main arena where B chromosome destiny is played. This suggests that B chromosome evolutionary success can lie on its gene content.
多年来，寄生 B 染色体一直被认为是遗传上无活性的元件。在这里，我们展示了草蜢 Eyprepocnemis plorans 的 B 染色体中存在十个蛋白编码基因。其中四个基因（CIP2A、GTPB6、KIF20A 和 MTG1）在 B 染色体上是完整的，而其余六个（CKAP2、CAP-G、HYI、MYCB2、SLIT 和 TOP2A）则是截断的。这五个基因（CIP2A、CKAP2、CAP-G、KIF20A 和 MYCB2）在携带 B 染色体的个体中显著上调，这与它们从 B 染色体上主动转录的预期相符。这一结论得到了三个截断基因（CKAP2、CAP-G 和 MYCB2）的支持，这些基因仅在存在于 B 染色体的区域上调。我们的结果表明，B 染色体并没有像以前认为的那样沉默。有趣的是，B 染色体上的五个活跃基因编码的功能与细胞分裂有关，这是 B 染色体命运发挥的主要领域。这表明 B 染色体的进化成功可能与其基因含量有关。
Zotero 插件