Sinitsky Maxim Yu, Matveeva Vera G, Asanov Maxim A, Ponasenko Anastasia V
Research Institute for Complex Issues of Cardiovascular Diseases, Sosnovy Boulevard 6, Kemerovo, 650002, Russia; The Federal Research Center of Coal and Coal Chemistry of SB RAS, Leningradsky Avenue 10, Kemerovo, 650065, Russia.
Research Institute for Complex Issues of Cardiovascular Diseases, Sosnovy Boulevard 6, Kemerovo, 650002, Russia.
Anal Biochem. 2018 Feb 15;543:128-131. doi: 10.1016/j.ab.2017.12.020. Epub 2017 Dec 13.
Adipose tissue is of interest in the context of its role in the pathogenesis of cardiovascular diseases. Modern experimental techniques require a well-purified RNA, but all the routine protocols for RNA extraction have a number of limitations in case of fatty tissues. Here we described a modified protocol for RNA extraction from human adipocytes based on routine column method. Suggested modifications optimized the sample preparation, lysis and washing lead to enhance RNA purity. We conclude that the current protocol for total RNA purification from adipocytes allows extracting a high-quality RNA devoid of fatty acids, organic solvents and salts contamination.
脂肪组织因其在心血管疾病发病机制中的作用而备受关注。现代实验技术需要高纯度的RNA，但所有常规的RNA提取方案在处理脂肪组织时都有一些局限性。在此，我们描述了一种基于常规柱法从人脂肪细胞中提取RNA的改良方案。建议的修改优化了样品制备、裂解和洗涤步骤，从而提高了RNA纯度。我们得出结论，当前从脂肪细胞中纯化总RNA的方案能够提取出不含脂肪酸、有机溶剂和盐污染的高质量RNA。
