Federal State Budgetary Institution "Research Institute for Complex Issues of Cardiovascular Disease", Sosnovyi bulvar, Kemerovo, Russian Federation.
PLoS One. 2021 Mar 18;16(3):e0248716. doi: 10.1371/journal.pone.0248716. eCollection 2021.
In coronary artery disease (CAD) the adipocytokine content in the heart fat depot is altered, but it has not been established whether these changes are associated with the degree of atherosclerotic damage to the coronary artery (CA). Were examined 84 patients with CAD, and according to the degree of atherosclerotic state based on the SYNTAX Score scale, were divided: 39 moderate (≤22 points), 20 severe (23-31 points) and 25 extremely severe (≥32 points). Biopsies of subcutaneous (SAT), epicardial (EAT) and perivascular adipose tissue (PVAT) were obtained during elective coronary artery bypass grafting (CABG). The expression of adipocytokine was determined using real-time PCR. The concentration of the studied adipocytokines in adipocyte culture medium was measured by ELISA. Statistical analysis was performed using logistic regression analysis. In the adipocytes of the cardiac depot of patients with CAD, an increase in the expression and secretion of leptin and IL-6 and a decrease in adiponectin, with a maximum manifestation in severe and extremely severe CA lesions, was observed. EAT adipocytes were characterized by minimal expression of the adiponectin gene maximal gene expression leptin and IL-6 compared to SAT and PVAT adipocytes.
在冠状动脉疾病（CAD）中，心脏脂肪库中的脂肪细胞因子含量发生改变，但尚未确定这些变化是否与冠状动脉（CA）粥样硬化损伤的程度有关。本研究检查了 84 例 CAD 患者，并根据 SYNTAX 评分量表上的粥样硬化状态程度进行了分组：39 例中度（≤22 分）、20 例重度（23-31 分）和 25 例极重度（≥32 分）。在择期冠状动脉旁路移植术（CABG）期间，从皮下（SAT）、心外膜（EAT）和血管周围脂肪组织（PVAT）中获取活检。使用实时 PCR 测定脂肪细胞因子的表达。通过 ELISA 测量脂肪细胞培养物中研究的脂肪细胞因子的浓度。使用逻辑回归分析进行统计分析。在 CAD 患者心脏脂肪库的脂肪细胞中，观察到瘦素和 IL-6 的表达和分泌增加，脂联素减少，在严重和极重度 CA 病变中表现最为明显。与 SAT 和 PVAT 脂肪细胞相比，EAT 脂肪细胞的脂联素基因表达最低，瘦素和 IL-6 基因表达最高。
