豆科植物 Bonduc 种仁的抗过敏和抗组胺作用：在哮喘治疗中的可能作用。
Antiallergic and antihistaminic actions of Ceasalpinia bonducella seeds: Possible role in treatment of asthma.
机构信息
Department of Pharmacognosy, Pravara Rural College of Pharmacy, Loni 413736, Maharashtra, India.
Department of Pharmacognosy, Pravara Rural College of Pharmacy, Loni 413736, Maharashtra, India.
出版信息
J Ethnopharmacol. 2018 Apr 24;216:251-258. doi: 10.1016/j.jep.2017.12.007. Epub 2017 Dec 14.
ETHNOPHARMACOLOGICAL RELEVANCE
Seed kernel of the plant Ceasalpinia bonducella Linn (Caesalpiniacaeae) are used for the treatment of asthma in folk medicine and ancient books.
AIM OF STUDY
To assess the pharmacological efficacy of the plant in asthma and to confine and describe the synthetic constituents from the seeds that are in charge of the action.
MATERIAL AND METHODS
The viability of petroleum ether, ethanol extract and ethyl acetate fraction from ethanol extract of C. bonducella seeds were screened for the treatment of asthma by various methods viz. effect of test drug on clonidine and haloperidol induced catalepsy, milk-induced leukocytosis and eosinophilia, mast cell stabilizing activity in mice and studies on smooth muscle preparation of guinea pig ileum (in-vitro). Column chromatography of active extract was done to pinpoint the active compound followed by structure elucidation by FTIR, GCMS and NMR spectroscopic methods.
RESULTS
Ethyl acetate fraction from ethanol extract of C. bonducella seeds exhibited antihistaminic activity at the dose of 50 and 100 mg/kg, inhibited clonidine-induced catalepsy but not haloperidol-induced catalepsy. Ethyl acetate fraction from ethanol extract significantly inhibited increased leukocyte and eosinophil count due to milk allergen and showed maximum protection against mast cell degranulation by clonidine. The results of guinea pig ileum indicated that the compound 2 methyl, 1 hexadecanol isolated from ethyl acetate fraction of ethanol extract relaxed significantly the ileum muscle strips pre-contracted by which suggests the involvement of β-agonists on the relaxation of the tissue. All the results are dose dependent. Active ethyl acetate fraction from ethanol extract showed presence of anti-asthmatic compound, 2-methyl, 1-hexadecanol.
CONCLUSION
The ethyl acetate fraction from ethanol extract of seeds of the plant C. bonducella can inhibit parameters linked to asthma disease.
植物决明子（豆科）的种仁在民间医学和古书中被用于治疗哮喘。
目的
评估该植物在哮喘中的药理作用，并确定和描述负责该作用的种子中的合成成分。
材料和方法
通过各种方法筛选决明子种子的石油醚、乙醇提取物和乙醇提取物的乙酸乙酯部分对哮喘的治疗作用，即测试药物对可乐定和氟哌啶醇诱导的僵住、牛奶诱导的白细胞增多和嗜酸性粒细胞增多、小鼠肥大细胞稳定活性以及豚鼠回肠平滑肌制剂的研究（体外）。对活性提取物进行柱层析，以确定活性化合物，然后通过 FTIR、GCMS 和 NMR 光谱方法阐明其结构。
结果
决明子种子乙醇提取物的乙酸乙酯部分在 50 和 100mg/kg 剂量下显示出抗组胺活性，抑制可乐定诱导的僵住，但不抑制氟哌啶醇诱导的僵住。乙醇提取物的乙酸乙酯部分显著抑制牛奶过敏原引起的白细胞和嗜酸性粒细胞计数增加，并对可乐定引起的肥大细胞脱颗粒显示出最大的保护作用。豚鼠回肠的结果表明，从乙醇提取物的乙酸乙酯部分分离出的化合物 2-甲基-1-十六烷醇显著松弛预先收缩的回肠肌肉条带，这表明β-激动剂参与了组织的松弛。所有结果均呈剂量依赖性。从乙醇提取物的乙酸乙酯部分显示出存在抗哮喘化合物 2-甲基-1-十六烷醇。
结论
该植物种子的乙醇提取物的乙酸乙酯部分可以抑制与哮喘病相关的参数。
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