Wróbel Tomasz, Dreger Mariola, Wielgus Karolina, Słomski Ryszard
Institute of Natural Fibres & Medicinal Plants, Wojska Polskiego 71b, 61-630, Poznan, Poland.
Department of Biochemistry and Biotechnology, Poznań University of Life Sciences, Dojazd 11, 60-632, Poznan, Poland.
Biotechnol Lett. 2018 Mar;40(3):445-454. doi: 10.1007/s10529-017-2492-1. Epub 2017 Dec 16.
Cannabinoids have considerable interest in the pharmaceutical industry. However, the production of medicines from hemp (Cannabis sativa L.) in most countries is restricted by law. Large-scale, field cultivation of hemp is difficult to control. Cannabinoid content in plants is variable and depends on multiple factors. Therefore, alternative methods of production have been investigated. The development of micropropagation techniques is a necessary step for genetic modification. Promising results have been obtained for certain narcotic genotypes. However, micropropagation of fibre types requires further research. Hemp can be genetically modified which may contribute to the breeding of new varieties in the future. Cell suspension cultures and hairy root cultures of hemp have been used to produce cannabinoids but obtaining cannabinoids from callus and cell suspension cultures has proved impossible. Adventitious roots can, however, deliver small amounts of these metabolites but production ceases over time and is too low for industrial applications.
大麻素在制药行业备受关注。然而，在大多数国家，从大麻（ Cannabis sativa L.）中生产药品受到法律限制。大麻的大规模田间种植难以控制。植物中的大麻素含量可变，且取决于多种因素。因此，人们研究了其他生产方法。微繁殖技术的发展是基因改造的必要步骤。对于某些麻醉基因型已取得了有前景的成果。然而，纤维类型的微繁殖还需要进一步研究。大麻可以进行基因改造，这可能有助于未来新品种的培育。大麻的细胞悬浮培养和毛状根培养已被用于生产大麻素，但事实证明，从愈伤组织和细胞悬浮培养物中获取大麻素是不可能的。然而，不定根可以产生少量这些代谢物，但产量会随着时间的推移而停止，且产量过低，无法用于工业应用。
