Das Rekhamani, Kretzschmar Tobias, Mieog Jos C
Faculty of Science and Engineering, Southern Cross University, Lismore, NSW 2480, Australia.
Plants (Basel). 2024 Sep 10;13(18):2544. doi: 10.3390/plants13182544.
Producing uniform for medicinal/recreational flower production through sexual propagation has been problematic, leading to dominance of clonal propagation from "mother plants" in the cannabinoid industry, which also faces significant limitations. tissue culture (TC) methods have been developed to overcome these challenges, but the long-term health and maintenance of explants in TC have been largely overlooked in previous studies. The current study focused on the development of an efficient and optimized micropropagation protocol covering the entire process, with a specific focus on the health and performance in the multiplication stage. Multiplication media were formulated hormone-free to avoid longer-term vitrification issues, resulting in single-main-shoot cultures rather than multiple-shoot cultures. This instigated the use of stage II explant types different from the standard shoot tips previously used for multiple shoot cultures. Multiplication media were further improved from the basal salt composition via nitrogen and calcium additives. The optimized protocol was used on eight diverse cultivars to test its applicability across various genetic backgrounds. Results indicated that the protocol was effective for conservation purposes across all cultivars and achieved good long-term multiplication rates for some but not all. The outcomes of this study mark a significant stride towards an efficient TC methodology ready for more comprehensive industrial applications.
通过有性繁殖生产用于药用/休闲花卉的均匀植株一直存在问题，这导致大麻素产业中来自“母株”的克隆繁殖占主导地位，而克隆繁殖也面临重大限制。已经开发出组织培养（TC）方法来克服这些挑战，但此前的研究很大程度上忽略了TC中外植体的长期健康和维护。当前的研究重点是开发一种高效且优化的微繁殖方案，涵盖整个过程，并特别关注增殖阶段的健康状况和表现。增殖培养基被配制为无激素，以避免长期的玻璃化问题，从而形成单主茎培养物而非多茎培养物。这促使使用与先前用于多茎培养的标准茎尖不同的II期外植体类型。通过添加氮和钙，从基础盐成分进一步改进了增殖培养基。优化后的方案应用于八个不同的品种，以测试其在各种遗传背景下的适用性。结果表明，该方案对于所有品种的保存目的都是有效的，并且对一些品种实现了良好的长期增殖率，但并非对所有品种都如此。这项研究的成果标志着朝着一种高效的TC方法迈出了重要一步，该方法可为更全面的工业应用做好准备。
