Englert Y, Delvigne A, Vekemans M, Lejeune B, Henlisz A, de Maertelaer G, Leroy F
In Vitro Fertilization Clinic, St. Pierre Hospital, Brussels, Belgium.
Fertil Steril. 1989 Apr;51(4):661-4. doi: 10.1016/s0015-0282(16)60617-9.
Sixty-six partners of either severely oligoasthenospermic or azoospermic men were treated by in vitro fertilization with donor sperm (IVF-D), usually (86%) after failure of artificial insemination with donor sperm. One hundred twenty-nine IVF trials were performed with either fresh (59%) or frozen-thawed (41%) donor sperm. Characteristics of sperm preparations were significantly lower in the frozen-thawed group (P less than 0.001). In the latter group, fertilization rates had slightly decreased (but without statistical significance); embryonic vitality scores and cryopreservable spare embryos had significantly decreased (P less than 0.05). However, cumulative ongoing pregnancy rates were strictly equivalent in both groups (40% after three trials). Frozen-thawed sperm thus can be substituted for fresh donor sperm without entailing impairment of the outcome of IVF, and this method must be preferred for its greater safety regarding transmission of human immunodeficiency virus.
66名严重少弱精子症或无精子症男性的伴侣接受了供精体外受精（IVF-D）治疗，通常（86%）是在供精人工授精失败后进行的。使用新鲜（59%）或冻融（41%）供精进行了129次IVF试验。冻融组精子制剂的特征显著更低（P<0.001）。在后者组中，受精率略有下降（但无统计学意义）；胚胎活力评分和可冷冻备用胚胎显著减少（P<0.05）。然而，两组的累积持续妊娠率严格相当（三次试验后为40%）。因此，冻融精子可替代新鲜供精，而不会损害IVF的结果，并且由于其在人类免疫缺陷病毒传播方面具有更高的安全性，这种方法更值得首选。
