Mahadevan M M, Trounson A O, Leeton J F
Fertil Steril. 1983 Sep;40(3):340-3. doi: 10.1016/s0015-0282(16)47297-3.
Frozen-thawed donor semen was compared with freshly ejaculated donor semen for in vitro fertilization of human oocytes in patients with infertile husbands. There was no significant difference in the fertilization rates between frozen-thawed (85%) and fresh spermatozoa (78%). Fertilization occurred with semen stored for as long as 27 months and with numbers of spermatozoa as low as 20,000/ml culture medium for insemination of oocytes. Pregnancy rates of embryos, fertilized with frozen-thawed and freshly ejaculated spermatozoa, were similar following embryo transfer.
将冻融供体精液与新鲜射出的供体精液用于丈夫不育患者的人卵母细胞体外受精，并进行比较。冻融精液（85%）和新鲜精子（78%）的受精率没有显著差异。储存长达27个月的精液以及用于卵母细胞授精的培养基中精子数量低至20000/ml时均可发生受精。胚胎移植后，用冻融精子和新鲜射出精子受精的胚胎妊娠率相似。
