Ge Nan, Chu Xiu-Mei, Xuan Yun-Peng, Ren Dun-Qiang, Wang Yongjie, Ma Kai, Gao Hui-Jiang, Jiao Wen-Jie
Department of Thoracic Surgery, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266000, P.R. China.
Department of Respiratory Medicine, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266000, P.R. China.
Oncol Lett. 2017 Dec;14(6):7538-7544. doi: 10.3892/ol.2017.7162. Epub 2017 Oct 10.
Lung cancer is a type of malignant tumor derived from the respiratory system, which is the leading cause of cancer-associated mortality worldwide, of which ~80% of cases are attributable to non-small cell lung cancer (NSCLC). A previous study demonstrated that 1α,25-Dihydroxyvitamin D (1α,25(OH)D), derived from the vitamin D metabolic pathway contributes an antitumor effect. Aberrant expression of the essential enzyme encoding genes, Cytochrome P450 Family 27 Subfamily A Member 1 (), Cytochrome P450 Family 27 Subfamily B Member 1 (), and Cytochrome P450 Family 24 Subfamily A Member 1 () may be associated with lung cancer. However, a lack of evidence exists concerning the association between , , expression and NSCLC. The aim of the present study was to investigate the functions of and expression in NSCLC. Lung cancer tissue and para-carcinoma control tissue were collected from patients with NSCLC. Reverse transcription-quantitative polymerase chain reaction was applied to analyze and mRNA expression in lung cancer tissues. An association analysis was performed between the aforementioned metabolic enzymes and patients with NSCLC age, gender, tumor node metastasis (TNM) stage, pathological type, differentiation and prognosis. and mRNA were upregulated in NSCLC compared with controls (P<0.05). However, no significant differences in expression were observed between NSCLC and control. In addition, expression was not associated with age, sex, smoking or TNM stage, but was associated with pathological type, differentiation and prognosis (P<0.05). expression was significantly associated with TNM stage, differentiation, and prognosis, but not age, sex, smoking or pathological type. In conclusion, and may be considered as independent prognostic factors of NSCLC and may be novel therapeutic targets to assist clinical diagnosis, treatment and prognosis of the disease.
肺癌是一种起源于呼吸系统的恶性肿瘤，是全球癌症相关死亡的主要原因，其中约80%的病例归因于非小细胞肺癌（NSCLC）。先前的一项研究表明，维生素D代谢途径产生的1α,25-二羟基维生素D（1α,25(OH)D）具有抗肿瘤作用。编码关键酶的基因，即细胞色素P450家族27亚家族A成员1（）、细胞色素P450家族27亚家族B成员1（）和细胞色素P450家族24亚家族A成员1（）的异常表达可能与肺癌有关。然而，关于、、表达与NSCLC之间的关联缺乏证据。本研究的目的是探讨和表达在NSCLC中的作用。从NSCLC患者中收集肺癌组织和癌旁对照组织。应用逆转录-定量聚合酶链反应分析肺癌组织中的和mRNA表达。对上述代谢酶与NSCLC患者的年龄、性别、肿瘤淋巴结转移（TNM）分期、病理类型、分化程度和预后进行关联分析。与对照组相比，NSCLC中的和mRNA上调（P<0.05）。然而，NSCLC与对照组之间的表达未观察到显著差异。此外，表达与年龄、性别、吸烟或TNM分期无关，但与病理类型、分化程度和预后有关（P<0.05）。表达与TNM分期、分化程度和预后显著相关，但与年龄、性别、吸烟或病理类型无关。总之，和可被视为NSCLC的独立预后因素，可能是辅助该疾病临床诊断、治疗和预后的新治疗靶点。
