Department of Zoology, Faculty of Science, Cairo University, Giza, Egypt.
Medical Science Department, Faculty of Dentistry, The British University in Egypt, Cairo, Egypt.
Biol Trace Elem Res. 2018 Sep;185(1):197-204. doi: 10.1007/s12011-017-1220-y. Epub 2017 Dec 18.
The uncontrolled releasing of hazardous wastes into Lake Qaroun was adversely impacting the aquatic environment and its biota. Therefore, this approach was designed to investigate the impact of these discharges on the reproductive and testicular function of Oreochromis niloticus. Several biomarkers were applied on the testicular tissues as metal bioaccumulation levels, histological examination with recording the ovotestis appearance and antioxidant status after 45 days of exposure to 0, 10, 20, & 30% of Lake Qaroun water (LQW). The bioaccumulated Cu, Mn, Fe, Pb, and Cd were unequally distributed in the testes but all these metals showed a significant concentration-dependent elevation among the studied groups. The exposure to LQW significantly increased the histological alterations in the testicular tissues as vacuolar degeneration, loss of tubular organization, and degeneration of Leydig cell. Based on the frequent appearance of histopathological abnormalities and ovotestis recording, the most deformed testicular tissues were observed in the 30% LQW-exposed groups. The ovotestis severity index (OSI) was significantly raised in LQW-exposed groups compared to the control group. The activities of superoxide dismutase, catalase as well as glutathione-reduced levels were significantly (p < 0.05) decreased in testes after chronic exposure to LQW. On the other pole, the activities of glutathione peroxidase enzyme and malondialdehyde formation were sharply increased. In view of the above, the exposure to LQW induced metal-related oxidative damage to the testicular tissues and impaired the male reproductive health. This study showed strong correlations between the anthropogenic activities around Lake Qaroun and the reproductive dysfunction in fish.
未经控制的危险废物排放到加龙湖（Qaroun Lake）中，对水生态环境及其生物群造成了不利影响。因此，本研究旨在调查这些排放物对尼罗罗非鱼（Oreochromis niloticus）生殖和睾丸功能的影响。在暴露于加龙湖（LQW）水 0、10、20 和 30%的第 45 天，将几种生物标志物应用于睾丸组织，包括金属生物累积水平、记录卵睾外观的组织学检查以及抗氧化状态。Cu、Mn、Fe、Pb 和 Cd 在睾丸中的生物累积分布不均，但所有这些金属在研究组中均表现出显著的浓度依赖性升高。暴露于 LQW 会显著增加睾丸组织的组织学变化，如空泡变性、管状组织丧失和 Leydig 细胞退化。根据组织病理学异常和卵睾记录的频繁出现，在 30%LQW 暴露组中观察到最变形的睾丸组织。与对照组相比，LQW 暴露组的卵睾严重指数（OSI）显著升高。在慢性暴露于 LQW 后，睾丸中超氧化物歧化酶、过氧化氢酶以及还原型谷胱甘肽的活性显著（p<0.05）降低，而谷胱甘肽过氧化物酶和丙二醛形成的活性急剧增加。综上所述，暴露于 LQW 会导致睾丸组织的金属相关氧化损伤，并损害雄性生殖健康。本研究表明，加龙湖周边的人为活动与鱼类生殖功能障碍之间存在很强的相关性。
