Department of Physics, School of Science, Nanjing University of Science and Technology , Nanjing 210094, China.
Nanjing Institute of Product Quality Inspection , Nanjing 210019, China.
ACS Appl Mater Interfaces. 2018 Jan 10;10(1):21-26. doi: 10.1021/acsami.7b14942. Epub 2017 Dec 20.
Bead-based assays have drawn more and more attention in biomedical fields. Herein, we proposed a novel approach to achieve multiplex analysis on a single porous hydrogel bead (PHB) with Raman dyes (RDs) encoded core-shell surface-enhanced Raman scattering (SERS) nanotags. Because of the amplified signal of RDs by core-shell metal structure of the nanotag and the high surface area to volume ratio (SVR) of the PHB, the sensitivity and linear dynamic range (LDR) of the as-proposed multiplex analysis method are significantly improved. We anticipate this approach to be widely used in the multiplex assays and biosensors.
基于珠体的分析方法在生物医学领域受到了越来越多的关注。在此，我们提出了一种新的方法，即在具有拉曼染料（RDs）编码核壳结构表面增强拉曼散射（SERS）纳米标签的单个多孔水凝胶珠（PHB）上实现多重分析。由于纳米标签的核壳金属结构对 RD 信号的放大以及 PHB 的高表面积与体积比（SVR），所提出的多重分析方法的灵敏度和线性动态范围（LDR）得到了显著提高。我们期望这种方法能够在多重分析和生物传感器中得到广泛应用。
