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蓝氏贾第鞭毛虫29.4千道尔顿结构蛋白的特性及其在腹吸盘的定位[已修正]

Characterization of a 29.4-kilodalton structural protein of Giardia lamblia and localization to the ventral disk [corrected].

作者信息

Aggarwal A, Adam R D, Nash T E

机构信息

Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892.

出版信息

Infect Immun. 1989 Apr;57(4):1305-10. doi: 10.1128/iai.57.4.1305-1310.1989.

DOI:10.1128/iai.57.4.1305-1310.1989
PMID:2925253
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC313266/
Abstract

The amino acid sequence of a 29.4-kilodalton [corrected] structural protein located in the ventral disk and axostyle of Giardia lamblia was determined. Clone lambda M16 from a mung bean expression library in lambda gt11 expressed a fusion protein recognized by three different isolate-specific antisera and sera from G. lamblia-infected gerbils. One of the three EcoRI fragments (M16; 1.26 kilobases) encoded the recognized protein. Sequence analysis revealed a single open reading frame of 813 base pairs. Two areas showed conservation of the positions of some amino acids. The abundance of arginine, glutamic acid, and threonine was increased. Two potential alpha-helical regions were deduced in the regions of repeats. Antisera to the M16 fusion protein reacted specifically with internal components of the ventral disk and axostyle, as well as Giardia fractions enriched for ventral disk structural proteins. An identical protein was recognized in different isolates by anti-M16, and a single identical band was recognized in Southern blots using the M16 1.26-kilobase fragment as a probe. Therefore, the 29.4-kilodaltion [corrected] protein appears to be highly conserved compared with variant surface proteins.

摘要

测定了位于蓝氏贾第鞭毛虫腹盘和轴柱中的一种29.4千道尔顿（校正后）结构蛋白的氨基酸序列。来自λgt11载体中的绿豆表达文库的λM16克隆表达了一种融合蛋白，该融合蛋白可被三种不同的分离株特异性抗血清以及来自感染蓝氏贾第鞭毛虫的沙鼠的血清识别。三个EcoRI片段之一（M16；1.26千碱基）编码了这种被识别的蛋白。序列分析揭示了一个813个碱基对的单一开放阅读框。两个区域显示出一些氨基酸位置的保守性。精氨酸、谷氨酸和苏氨酸的丰度增加。在重复区域推断出两个潜在的α螺旋区域。针对M16融合蛋白的抗血清与腹盘和轴柱的内部成分以及富含腹盘结构蛋白的贾第鞭毛虫组分发生特异性反应。抗M16在不同分离株中识别出相同的蛋白，并且使用M16 1.26千碱基片段作为探针在Southern印迹中识别出一条单一的相同条带。因此，与可变表面蛋白相比，这种29.4千道尔顿（校正后）的蛋白似乎高度保守。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad7d/313266/3701b796573c/iai00064-0311-a.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad7d/313266/0c3f2dd026fe/iai00064-0308-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad7d/313266/c770dfa86a8c/iai00064-0309-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad7d/313266/1ec2f80a24e1/iai00064-0310-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad7d/313266/d13e84d27c8e/iai00064-0310-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad7d/313266/3701b796573c/iai00064-0311-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad7d/313266/7ef40ef1caae/iai00064-0307-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad7d/313266/eb9ea5070a50/iai00064-0308-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad7d/313266/0c3f2dd026fe/iai00064-0308-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad7d/313266/c770dfa86a8c/iai00064-0309-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad7d/313266/1ec2f80a24e1/iai00064-0310-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad7d/313266/d13e84d27c8e/iai00064-0310-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad7d/313266/3701b796573c/iai00064-0311-a.jpg

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本文引用的文献

1
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J Cell Sci. 1981 Feb;47:167-85. doi: 10.1242/jcs.47.1.167.
2
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J Cell Sci. 1981 Feb;47:139-66. doi: 10.1242/jcs.47.1.139.
3
Antigenic analysis of Giardia lamblia from Afghanistan, Puerto Rico, Ecuador, and Oregon.来自阿富汗、波多黎各、厄瓜多尔和俄勒冈州的蓝氏贾第鞭毛虫的抗原分析。
贾第虫的两个细胞核都有基因组的完整拷贝，并在胞质分裂时均等分配。
Eukaryot Cell. 2002 Apr;1(2):191-9. doi: 10.1128/EC.1.2.191-199.2002.
4
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Clin Microbiol Rev. 2001 Jul;14(3):447-75. doi: 10.1128/CMR.14.3.447-475.2001.
5
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Clin Microbiol Rev. 2000 Jan;13(1):35-54, table of contents. doi: 10.1128/CMR.13.1.35.
6
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J Cell Biol. 1993 May;121(4):837-45. doi: 10.1083/jcb.121.4.837.
7
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8
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9
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4
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5
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6
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Science. 1983 Nov 18;222(4625):778-82. doi: 10.1126/science.6356359.
7
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9
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J Cell Biol. 1966 May;29(2):317-32. doi: 10.1083/jcb.29.2.317.