Seegers Christel L C, Tepper Pieter G, Setroikromo Rita, Quax Wim J
Department of Chemical and Pharmaceutical Biology, Groningen Research Institute of Pharmacy, University of Groningen, Groningen, Netherlands.
Planta Med. 2018 May;84(8):544-550. doi: 10.1055/s-0043-123938. Epub 2017 Dec 18.
Deoxypodophyllotoxin is present in the roots of . This compound is cytotoxic on its own, but it can also be converted into podophyllotoxin, which is in high demand as a precursor for the important anticancer drugs etoposide and teniposide. In this study, deoxypodophyllotoxin is extracted from roots by supercritical carbon dioxide extraction. The process is simple and scalable. The supercritical carbon dioxide method extracts 75 - 80% of the total deoxypodophyllotoxin content, which is comparable to a single extraction by traditional Soxhlet. However, less polar components are extracted. The activity of the supercritical carbon dioxide extract containing deoxypodophyllotoxin was assessed by demonstrating that the extract arrests A549 and HeLa cells in the G/M phase of the cell cycle. We conclude that biologically active deoxypodophyllotoxin can be extracted from by supercritical carbon dioxide extraction. The method is solvent free and more sustainable compared to traditional methods.
脱氧鬼臼毒素存在于……的根部。这种化合物本身具有细胞毒性，但它也可以转化为鬼臼毒素，作为重要抗癌药物依托泊苷和替尼泊苷的前体，其需求量很大。在本研究中，通过超临界二氧化碳萃取法从……根部提取脱氧鬼臼毒素。该过程简单且可扩展。超临界二氧化碳法提取了总脱氧鬼臼毒素含量的75%-80%，这与传统索氏提取法单次提取的量相当。然而，极性较小的成分被提取出来。通过证明含有脱氧鬼臼毒素的超临界二氧化碳提取物能使A549和HeLa细胞停滞在细胞周期的G/M期，对其活性进行了评估。我们得出结论，可通过超临界二氧化碳萃取法从……中提取具有生物活性的脱氧鬼臼毒素。与传统方法相比，该方法无溶剂且更具可持续性。
