Department of Biomedical Science, BK21 PLUS Centre for Creative Biomedical Scientists, Chonnam National University Medical School, 160 Baekseo-ro, Gwangju 61469, Republic of Korea; Department of Polymer Science and Engineering, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, Republic of Korea.
Department of Nuclear Medicine, Chonnam National University Medical School, Gwangju 61469, Republic of Korea.
Carbohydr Polym. 2018 Feb 1;181:27-33. doi: 10.1016/j.carbpol.2017.10.042. Epub 2017 Oct 12.
The development of biologically targeted contrast agents for X-ray computed tomography (CT) imaging remains a major challenge. Here, we investigated a green chemistry-based synthesis of lymph node-targeted mannan-capped gold nanoparticles (M-GNPs) as a CT contrast agent. In this study, mannan was used as a reducing and stabilizing agent for gold nanoparticles (AuNPs). M-GNPs were readily internalized by antigen-presenting cells (APCs) through mannose receptors-mediated endocytosis. The M-GNPs, which had a spherical morphology, had an average diameter of 9.18±0.71nm and surface plasmon resonance (SPR) absorption spectra with maximal absorption at 522nm. The M-GNPs displayed a concentration-based X-ray attenuation property with a maximum Hounsfield unit (HU) value of 303.2±10.83. The local administration of M-GNPs led to significantly enhanced X-ray contrast for the imaging of popliteal lymph nodes. These findings demonstrated that M-GNPs can be used as biologically targeted contrast agents for CT imaging.
用于 X 射线计算机断层扫描（CT）成像的生物靶向对比剂的开发仍然是一个主要挑战。在这里，我们研究了基于绿色化学的用于 CT 造影剂的淋巴靶向甘露聚糖包覆金纳米颗粒（M-GNPs）的合成。在这项研究中，甘露聚糖被用作金纳米颗粒（AuNPs）的还原剂和稳定剂。M-GNPs 通过甘露糖受体介导的内吞作用被抗原呈递细胞（APCs）轻易地内化。M-GNPs 具有球形形态，平均直径为 9.18±0.71nm，表面等离子体共振（SPR）吸收光谱在 522nm 处具有最大吸收。M-GNPs 表现出基于浓度的 X 射线衰减特性，最大亨氏单位（HU）值为 303.2±10.83。M-GNPs 的局部给药导致对隐窝淋巴结成像的 X 射线对比度显著增强。这些发现表明，M-GNPs 可用作 CT 成像的生物靶向对比剂。
