Department of Advanced Organic Materials and Textile Engineering System, Chungnam National University, Daejeon 34134, South Korea.
Department of Veterinary Medicine, Chungnam National University, Daejeon 43134, South Korea.
Carbohydr Polym. 2018 Feb 1;181:579-586. doi: 10.1016/j.carbpol.2017.11.109. Epub 2017 Dec 2.
A thermo-sensitive methylcellulose (MC) hydrogel containing silver oxide nanoparticles (NPs) was prepared via one-pot synthesis in which a silver acetate precursor salt (CHCOOAg) induces a salt-out effect in the MC solution. The silver oxide NPs were synthesized in situ from Ag ions during the MC hydrogelation, and the residual CHCOO ions decreased the gelation temperature of the MC solution through the salt-out effect. The gelation behavior of the MC solution varied according to the CHCOOAg content and was monitored. Also, the formation and structure of the silver oxide NPs in the MC hydrogel was confirmed. From the results, silver oxide NPs was successfully incorporated in MC hydrogels, simultaneously, acetate ion which was counter ion of Ag was affected gelation behavior of Ag. Finally, the antimicrobial activity and wound healing effect was examined using the shaking flask method and burn wound test, respectively. The MC hydrogel with silver oxide NPs showed excellent antimicrobial activity and burn wound healing. Therefore, this thermo-responsive MC hydrogel has great potential as an injectable hydrogel for wound regeneration.
一种温敏性的含有氧化银纳米粒子（NPs）的甲基纤维素（MC）水凝胶是通过一锅合成法制备的，其中醋酸银前体盐（CHCOOAg）在 MC 溶液中诱导盐析效应。氧化银 NPs 是在 MC 水凝胶化过程中由 Ag 离子原位合成的，残留的 CHCOO 离子通过盐析效应降低了 MC 溶液的凝胶化温度。MC 溶液的凝胶化行为随 CHCOOAg 含量的变化而变化，并进行了监测。此外，还证实了 MC 水凝胶中氧化银 NPs 的形成和结构。结果表明，氧化银 NPs 成功地掺入了 MC 水凝胶中，同时，Ag 的抗衡离子醋酸根离子影响了 Ag 的凝胶化行为。最后，分别采用振摇瓶法和烧伤创面试验考察了其抗菌活性和创面愈合效果。含有氧化银纳米粒子的 MC 水凝胶表现出优异的抗菌活性和烧伤创面愈合效果。因此，这种温敏性 MC 水凝胶作为一种可注射水凝胶在创伤再生方面具有很大的应用潜力。
