G4941K substitution in the pore-lining S6 helix of the skeletal muscle ryanodine receptor increases RyR1 sensitivity to cytosolic and luminal Ca.

The ryanodine receptor ion channel RyR1 is present in skeletal muscle and has a large cytoplasmic N-terminal domain and smaller C-terminal pore-forming domain comprising six transmembrane helices, a pore helix, and a selectivity filter. The RyR1 S6 pore-lining helix has two conserved glycines, Gly-4934 and Gly-4941, that facilitate RyR1 channel gating by providing S6 flexibility and minimizing amino acid clashes. Here, we report that substitution of Gly-4941 with Asp or Lys results in functional channels as indicated by caffeine-induced Ca release response in HEK293 cells, whereas a low response of the corresponding Gly-4934 variants suggested loss of function. Following purification, the RyR1 mutants G4934D, G4934K, and G4941D did not noticeably conduct Ca in single-channel measurements. Gly-4941 replacement with Lys resulted in channels having reduced K conductance and reduced selectivity for Ca compared with wildtype. RyR1-G4941K did not fully close at nanomolar cytosolic Ca concentrations and nearly fully opened at 2 μm cytosolic or sarcoplasmic reticulum luminal Ca, and Ca- and voltage-dependent regulation of RyR1-G4941K mutant channels was demonstrated. Computational methods and single-channel recordings indicated that the open G4941K variant results in the formation of a salt bridge to Asp-4938. In contrast, wildtype RyR1 was closed and not activated by luminal Ca at low cytosolic Ca levels. A model suggested that luminal Ca activates RyR1 by accessing a recently identified cytosolic Ca-binding site in the open channel as the Ca ions pass through the pore.