Okpuzor J, Ogbunugafor H A, Okafor U, Sofidiya M O
Department of Cell Biology and Genetics, Faculty of Science, University of Lagos, Akoka, Lagos.
Applied Biochemistry Department, Faculty of Natural Sciences, Nnamdi Azikiwe University, Awka, Nigeria.
EXCLI J. 2010 Feb 1;9:17-28. eCollection 2010.
This study aims to identify the types of proteins in malted and dry Bambara groundnut seeds and through a comparative analysis, identify similarities and their known uses. Dry viable bambara seed was stored for five days to malt. The proteins in the dry and malted seed were subsequently extracted in potassium phosphate buffer pH 7.0 and precipitated with saturated ammonium sulphate. MudPit (multidimensional protein identification technology) and LC-MALDI TOF-TOF (liquid chromatography - matrix-assisted laser desorption ionization tandem time-of-flight) mass spectrometry were thereafter used to identify the different types of proteins. A total of ten and twelve different types of proteins present in other legume species were identified in the malted and dry seeds respectively from the 214 peptides isolated after searching 586 proteins of the genus . Seed storage protein B and vicilin were observed to be the major proteins common to both malted and dry seeds and are similar to . Some of the other proteins observed showed amino acid sequence homology with and species. The following proteins BV1, Heat shock and Bowman-Birk Inhibitor (a protease), were observed only in the malted state. This information may enhance the appreciation of the nutritional and health benefits of the seed.
本研究旨在鉴定发芽和干燥的 Bambara 花生种子中的蛋白质类型，并通过比较分析确定其相似性及其已知用途。将干燥的有活力的 Bambara 种子储存五天以使其发芽。随后在 pH 7.0 的磷酸钾缓冲液中提取干燥种子和发芽种子中的蛋白质，并用饱和硫酸铵沉淀。此后，使用 MudPit（多维蛋白质鉴定技术）和 LC-MALDI TOF-TOF（液相色谱 - 基质辅助激光解吸电离串联飞行时间）质谱法鉴定不同类型的蛋白质。在搜索该属的 586 种蛋白质后分离出的 214 种肽段中，分别在发芽种子和干燥种子中鉴定出了其他豆科物种中存在的总共十种和十二种不同类型的蛋白质。种子储存蛋白 B 和豌豆球蛋白被观察到是发芽种子和干燥种子共有的主要蛋白质，并且与……相似。观察到的一些其他蛋白质与……物种显示出氨基酸序列同源性。以下蛋白质 BV1、热休克蛋白和鲍曼 - 伯克抑制剂（一种蛋白酶）仅在发芽状态下被观察到。这些信息可能会增强对该种子营养和健康益处的认识。
