Key Laboratory of Groundwater Resources and Environment, Ministry of Education, Jilin University, Changchun, 130021, China.
College of Environment and Resources, Jilin University, Changchun, 130021, China.
Environ Sci Pollut Res Int. 2018 Mar;25(7):6497-6513. doi: 10.1007/s11356-017-0876-3. Epub 2017 Dec 18.
Virus is one of the most potentially harmful microorganisms in groundwater. In this paper, the effects of hydrodynamic and hydrogeochemical conditions on the transportation of the colloidal virus considering managed aquifer recharge were systematically investigated. Escherichia coli phage, vB_EcoM-ep3, has a broad host range and was able to lyse pathogenic Escherichia coli. Bacteriophage with low risk to infect human has been found extensively in the groundwater environment, so it is considered as a representative model of groundwater viruses. Laboratory studies were carried out to analyze the transport of the Escherichia coli phage under varying conditions of pH, ionic strength, cation valence, flow rate, porous media, and phosphate buffer concentration. The results indicated that decreasing the pH will increase the adsorption of Escherichia coli phage. Increasing the ionic strength, either Na or Ca, will form negative condition for the migration of Escherichia coli phage. A comparison of different cation valence tests indicated that changes in transport and deposition were more pronounced with divalent Ca than monovalent Na. As the flow rate increases, the release of Escherichia coli phage increases and the retention of Escherichia coli phage in the aquifer medium reduces. Changes in porous media had a significant effect on Escherichia coli phage migration. With increase of phosphate buffer concentration, the suspension stability and migration ability of Escherichia coli phage are both increased. Based on laboratory-scale column experiments, a one-dimensional transport model was established to quantitatively describe the virus transport in saturated porous medium.
病毒是地下水中最具潜在危害的微生物之一。本文系统研究了水动力和水文地球化学条件对考虑含水层人工补给时胶体病毒运移的影响。大肠杆菌噬菌体 vB_EcoM-ep3 宿主范围广，能够裂解致病性大肠杆菌。在地下水环境中广泛发现了对人类感染风险低的噬菌体，因此被认为是地下水病毒的代表性模型。进行了实验室研究，以分析在不同 pH 值、离子强度、阳离子价态、流速、多孔介质和磷酸盐缓冲液浓度条件下大肠杆菌噬菌体的迁移。结果表明，降低 pH 值会增加大肠杆菌噬菌体的吸附。增加离子强度，无论是 Na 还是 Ca，都会对大肠杆菌噬菌体的迁移形成不利条件。不同阳离子价态测试的比较表明，与单价 Na 相比，二价 Ca 的运输和沉积变化更为明显。随着流速的增加，大肠杆菌噬菌体的释放增加，而在含水层介质中对大肠杆菌噬菌体的保留减少。多孔介质的变化对大肠杆菌噬菌体的迁移有显著影响。随着磷酸盐缓冲液浓度的增加，大肠杆菌噬菌体的悬浮稳定性和迁移能力都得到了提高。基于实验室规模的柱实验，建立了一个一维传输模型来定量描述饱和多孔介质中病毒的传输。
