Shim Jae Won, Bae In-Ho, Park Dae Sung, Lee So-Youn, Jang Eun-Jae, Lim Kyung-Seob, Park Jun-Kyu, Kim Ju Han, Jeong Myung Ho
1 The Cardiovascular Convergence Research Center of Chonnam National University Hospital Designated by Korea Ministry of Health and Welfare, Gwangju, Republic of Korea.
2 Korea Cardiovascular Stent Research Institute, Jangsung, Korea.
J Biomater Appl. 2018 Mar;32(8):1083-1089. doi: 10.1177/0885328217748465. Epub 2017 Dec 19.
The first two authors contributed equally to this study. Bioactivity and cell adhesion properties are major factors for fabricating medical devices such as coronary stents. The aim of this study was to evaluate the advantages of atmospheric-pressure plasma jet in enhancing the biocompatibility and endothelial cell-favorites. The experimental objects were divided into before and after atmospheric-pressure plasma jet treatment with the ratio of nitrogen:argon = 3:1, which is similar to air. The treated surfaces were basically characterized by means of a contact angle analyzer for the activation property on their surfaces. The effect of atmospheric-pressure plasma jet on cellular response was examined by endothelial cell adhesion and XTT analysis. It was difficult to detect any changeable morphology after atmospheric-pressure plasma jet treatment on the surface. The roughness was increased after atmospheric-pressure plasma jet treatment compared to nonatmospheric-pressure plasma jet treatment (86.781 and 7.964 nm, respectively). The X-ray photoelectron spectroscopy results showed that the surface concentration of the C-O groups increased slightly from 6% to 8% after plasma activation. The contact angle dramatically decreased in the atmospheric-pressure plasma jet treated group (22.6 ± 15.26°) compared to the nonatmospheric-pressure plasma jet treated group (72.4 ± 15.26°) ( n = 10, p < 0.05). The effect of the increment in hydrophilicity due to the atmospheric-pressure plasma jet on endothelial cell migration and proliferation was 85.2% ± 12.01% and 34.2% ± 2.68%, respectively, at 7 days, compared to the nonatmospheric-pressure plasma jet treated group (58.2% ± 11.44% in migration, n = 10, p < 0.05). Taken together, the stent surface could easily obtain a hydrophilic property by the atmospheric-pressure plasma jet method. Moreover, the atmospheric-pressure plasma jet might affect re-endothelialization after stenting.
前两位作者对本研究贡献相同。生物活性和细胞黏附特性是制造冠状动脉支架等医疗器械的主要因素。本研究的目的是评估大气压等离子体射流在增强生物相容性和内皮细胞亲和力方面的优势。实验对象分为大气压等离子体射流处理前和处理后两组，氮气与氩气的比例为3:1，类似于空气。通过接触角分析仪对处理后的表面进行基本表征，以了解其表面的活化特性。通过内皮细胞黏附和XTT分析研究大气压等离子体射流对细胞反应的影响。大气压等离子体射流处理后，表面难以检测到任何可变化的形态。与非大气压等离子体射流处理相比，大气压等离子体射流处理后粗糙度增加（分别为86.781和7.964纳米）。X射线光电子能谱结果表明，等离子体活化后，C-O基团的表面浓度从6%略有增加至8%。与非大气压等离子体射流处理组（72.4±15.26°）相比，大气压等离子体射流处理组的接触角显著降低（22.6±15.26°）（n = 10，p < 0.05）。与非大气压等离子体射流处理组相比，大气压等离子体射流引起的亲水性增加对内皮细胞迁移和增殖的影响在7天时分别为85.2%±12.01%和34.2%±2.68%（迁移率为58.2%±11.44%，n = 10，p < 0.05）。综上所述，通过大气压等离子体射流方法，支架表面可轻松获得亲水性。此外，大气压等离子体射流可能会影响支架置入后的再内皮化。
