Saito M, Yamauchi Y, Inomata K, Kottkamp W
JASCO, Japan Spectroscopic Co., Ltd., Tokyo.
J Chromatogr Sci. 1989 Feb;27(2):79-85. doi: 10.1093/chromsci/27.2.79.
Enrichment of tocopherol by coupled supercritical fluid extraction/preparative supercritical fluid chromatography is described. Wheat germ powder is used as the starting material and is subjected to supercritical fluid extraction with carbon dioxide. The extracted oil containing tocopherols is concentrated and trapped on a silica gel column by reducing the pressure of carbon dioxide. The trapped oil is then eluted and separated on a silica gel column of 20 mm i.d. x 20 mm length. The column effluent is fractionated by monitoring UV absorption at 290 nm. With this method, tocopherol content of the wheat germ is enriched 100-fold.
描述了通过超临界流体萃取/制备型超临界流体色谱联用对生育酚进行富集的方法。以小麦胚芽粉为起始原料，用二氧化碳进行超临界流体萃取。将含有生育酚的萃取油浓缩，并通过降低二氧化碳压力将其捕集在硅胶柱上。然后将捕集的油在内径20mm×长度20mm的硅胶柱上洗脱并分离。通过监测290nm处的紫外吸收对柱流出物进行分馏。用这种方法，小麦胚芽中的生育酚含量提高了100倍。
