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小麦（普通小麦）籽粒和幼苗中生育酚及类异戊二烯醌的测定

The determination of tocopherols and isoprenoid quinones in the grain and seedlings of wheat (Triticum vulgare).

作者信息

Hall G S, Laidman D L

出版信息

Biochem J. 1968 Jul;108(3):465-73. doi: 10.1042/bj1080465.

DOI:10.1042/bj1080465
PMID:5667256
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1198832/
Abstract
  1. A comparison is made of several procedures for the extraction of tocopherols and isoprenoid quinones from plant tissues. 2. Gradient-elution column chromatography on acid-washed alumina efficiently separates the isoprenoid quinones and tocopherols into groups that can then be assayed spectrophotometrically or, with the tocopherols, separated into their individual components and determined by gas-liquid chromatography. 3. This improved analytical procedure was used to study the distribution of the tocopherols and of ubiquinone in the ungerminated wheat grain.
摘要
  1. 对从植物组织中提取生育酚和类异戊二烯醌的几种方法进行了比较。2. 在酸洗过的氧化铝上进行梯度洗脱柱色谱，可有效地将类异戊二烯醌和生育酚分离成不同的组，然后可以通过分光光度法进行测定，或者对于生育酚，将其分离成各个组分并通过气液色谱法进行测定。3. 采用这种改进的分析方法研究了未发芽小麦籽粒中生育酚和泛醌的分布。

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1
The determination of tocopherols and isoprenoid quinones in the grain and seedlings of wheat (Triticum vulgare).小麦（普通小麦）籽粒和幼苗中生育酚及类异戊二烯醌的测定
Biochem J. 1968 Jul;108(3):465-73. doi: 10.1042/bj1080465.
2
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引用本文的文献

1
The pattern and control of isoprenoid quinone and tocopherol metabolism in the germinating grain of wheat (Triticum vulgare).小麦（普通小麦）萌发种子中类异戊二烯醌和生育酚代谢的模式与调控。
Biochem J. 1968 Jul;108(3):475-82. doi: 10.1042/bj1080475.
2
Distribution of tocopherols within the corn kernel.生育酚在玉米粒中的分布。
J Am Oil Chem Soc. 1970 Sep;47(9):337-9. doi: 10.1007/BF02638997.
3
Gas chromatography of the fat-soluble vitamins: a review.脂溶性维生素的气相色谱法：综述
J Am Oil Chem Soc. 1972 Nov;49(11):619-33. doi: 10.1007/BF02609374.
4
Rapid method for the quantitative determination of individual tocopherols in oils and fats.油脂中各生育酚定量测定的快速方法。
J Am Oil Chem Soc. 1976 Dec;53(12):732-6. doi: 10.1007/BF02635472.

本文引用的文献

1
The isolation of n-octacosanol from wheat wax.从小麦蜡中分离出正二十八烷醇。
Biochem J. 1933;27(6):1889-93. doi: 10.1042/bj0271889.
2
Analysis and distribution of tocopherols and quinones in the pea plant.分析和豌豆植株中生育酚和醌类的分布。
Plant Physiol. 1967 Jun;42(6):851-8. doi: 10.1104/pp.42.6.851.
3
Metabolic Changes Associated with the Germination of Corn. I. Changes in Weight and Metabolites and their Redistribution in the Embryo Axis, Scutellum, and Endosperm.与玉米萌发相关的代谢变化。I. 重量和代谢物的变化及其在胚轴、盾片和胚乳中的重新分布。
Plant Physiol. 1964 Sep;39(5):735-40. doi: 10.1104/pp.39.5.735.
4
Gibberellic Acid Controlled Synthesis of alpha-Amylase in Barley Endosperm.赤霉素对大麦胚乳中α-淀粉酶合成的调控
Plant Physiol. 1964 May;39(3):413-5. doi: 10.1104/pp.39.3.413.
5
Internal Distribution of Coenzyme Q in Higher Plants.辅酶Q在高等植物中的内部分布
Plant Physiol. 1959 Mar;34(2):128-31. doi: 10.1104/pp.34.2.128.
6
Gas-liquid chromatographic fractionation of natural triglyceride mixtures by carbon number.通过碳数对天然甘油三酯混合物进行气液色谱分离。
Can J Biochem Physiol. 1962 May;40:679-86.
7
Coenzyme Q. IX. Coenzyme Q9 and Q10 content of dietary components.辅酶Q。IX。膳食成分中的辅酶Q9和Q10含量。
Arch Biochem Biophys. 1959 Dec;85:474-7. doi: 10.1016/0003-9861(59)90513-2.
8
Substance SC (ubichromenol): a naturally-occurring cyclic isomeride of ubiquinone-50.物质SC（泛色烯醇）：一种天然存在的辅酶Q-50的环状异构体。
Biochem J. 1960 Mar;74(3):541-9. doi: 10.1042/bj0740541.
9
PREPARATION OF FATTY ACID METHYL ESTERS AND DIMETHYLACETALS FROM LIPIDS WITH BORON FLUORIDE--METHANOL.用氟化硼 - 甲醇从脂质制备脂肪酸甲酯和二甲基缩醛
J Lipid Res. 1964 Oct;5:600-8.
10
COENZYME Q (UBIQUINONE).辅酶Q（泛醌）。
Adv Enzymol Relat Subj Biochem. 1963;25:275-328. doi: 10.1002/9780470122709.ch5.