Erkoç F U, Ozsar S, Güven B, Kalkandelen G, Uğrar E
Turkish Atomic Energy Authority, Lalahan Nuclear Research Institute of Animal Health, Ankara.
J Chromatogr Sci. 1989 Feb;27(2):86-90. doi: 10.1093/chromsci/27.2.86.
In the present work, a practical, rapid, reliable and isocratic reversed-phase high-performance liquid chromatographic (HPLC) method is described for the qualitative and quantitative analysis of estriol, estradiol-17 beta, estrone, testosterone, and progesterone. Chromatographic separation is complete in 16 min using a mobile phase of 50% acetonitrile (v/v) in water. The order of elution is estriol, testosterone, estradiol-17 beta, estrone, and progesterone; retention times are 2.5, 5.5, 5.6, 6.9, 16.3 min, respectively. Absorbance maxima of individual steroids is the limiting factor in quantitative determination. The recommended wavelengths for UV monitoring are E3 214, E2 280, T 254, E1 214, and P4 254 nm.
在本研究中，描述了一种实用、快速、可靠的等度反相高效液相色谱（HPLC）方法，用于雌三醇、17β-雌二醇、雌酮、睾酮和孕酮的定性和定量分析。使用含50%乙腈（v/v）的水作为流动相，16分钟内即可完成色谱分离。洗脱顺序为雌三醇、睾酮、17β-雌二醇、雌酮和孕酮；保留时间分别为2.5、5.5、5.6、6.9、16.3分钟。各甾体的最大吸收波长是定量测定的限制因素。紫外监测的推荐波长为：雌三醇214nm、雌二醇280nm、睾酮254nm、雌酮214nm、孕酮254nm。
